Stumbling across the Same Phage: Comparative Genomics of Widespread Temperate Phages Infecting the Fish Pathogen Vibrio anguillarum

Viruses
Panos G KalatzisMathias Middelboe

Abstract

Nineteen Vibrio anguillarum-specific temperate bacteriophages isolated across Europe and Chile from aquaculture and environmental sites were genome sequenced and analyzed for host range, morphology and life cycle characteristics. The phages were classified as Siphoviridae with genome sizes between 46,006 and 54,201 bp. All 19 phages showed high genetic similarity, and 13 phages were genetically identical. Apart from sporadically distributed single nucleotide polymorphisms (SNPs), genetic diversifications were located in three variable regions (VR1, VR2 and VR3) in six of the phage genomes. Identification of specific genes, such as N6-adenine methyltransferase and lambda like repressor, as well as the presence of a tRNAArg, suggested a both mutualistic and parasitic interaction between phages and hosts. During short term phage exposure experiments, 28% of a V. anguillarum host population was lysogenized by the temperate phages and a genomic analysis of a collection of 31 virulent V. anguillarum showed that the isolated phages were present as prophages in >50% of the strains covering large geographical distances. Further, phage sequences were widely distributed among CRISPR-Cas arrays of publicly available sequenced Vibrios. The ...Continue Reading

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Citations

Oct 24, 2017·Viruses·Mathias Middelboe, Corina P D Brussaard
Mar 25, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Ville HoikkalaLotta-Riina Sundberg
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Jul 4, 2018·Scientific Reports·Daniel CastilloMathias Middelboe
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Jun 27, 2020·Current Microbiology·Xinran ZhangAndrew McMinn
May 15, 2021·Environmental Microbiology·Castillo DanielMiddelboe Mathias

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Datasets Mentioned

BETA
KY658680

Methods Mentioned

BETA
restriction-modification
Polymerase Chain Reaction
PCR
protein folding

Software Mentioned

ARAGORN
CRISPR Finder
Geneious Tree Builder
Glimmer
Rapid Annotation Subsystem Technology (
Geneious
PHAST

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