Stunned peri-infarct canine myocardium is characterized by degradation of troponin T, not troponin I

Cardiovascular Research
David A ColantonioKarin Przyklenk

Abstract

Degradation of cardiac troponin I (cTnI) has been proposed to represent the underlying molecular mechanism responsible for post-ischemic contractile dysfunction of viable but 'stunned' myocardium. However, this concept is largely derived from models of brief, sublethal ischemia essentially devoid of necrosis, and there is speculation that defects in cTnI may be model-dependent. Accordingly, our primary aim was to evaluate the integrity of cardiac troponins-i.e., cTnI, as well as cTnT and cTnC-in viable but stunned peri-infarct tissue. In addition, we addressed the as-yet unexplored issue of whether the profound reduction of infarct size evoked by brief preconditioning ischemia (PC) was accompanied by a favorable attenuation in ischemia/reperfusion-induced degradation of cTnI, cTnT or cTnC in the remaining viable subepicardium. Anesthetized open-chest dogs received 10 min of PC ischemia or a comparable control period, followed by 1 h of sustained coronary occlusion and 3 h of reperfusion. Subepicardial biopsies from the center of the soon-to-be ischemic territory were obtained at baseline and at 30 min and 3 h post-reflow, and myofilament protein integrity (intact cTnI, cTnT and cTnC, as well as degradation bands and covalent co...Continue Reading

Citations

Jan 18, 2006·Heart Failure Reviews·Martin M LeWinter
Apr 2, 2008·The Journal of Biological Chemistry·Kittipong TachampaPieter P de Tombe
May 1, 2012·Cardiovascular Research·Hiroyuki Sorimachi, Yasuko Ono
May 15, 2012·American Journal of Respiratory Cell and Molecular Biology·Hasan A AhmadClifford R Greyson
Apr 3, 2007·Molecular Diagnosis & Therapy·Melanie Y White, Jennifer E Van Eyk
Dec 11, 2007·Journal of Molecular and Cellular Cardiology·Clifford R GreysonHasan Ahmad
Jun 1, 2008·Proteomics. Clinical Applications·Wenhai JinAnne M Murphy
Dec 13, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Donald E KuhnChandan K Sen
Nov 1, 2018·Physiological Reviews·Jolanda van der Velden, Ger J M Stienen
Aug 9, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Eias E JweiedPieter P de Tombe
Mar 2, 2005·Progress in Cardiovascular Diseases·Jason L McDonough, Jennifer E Van Eyk
Mar 2, 2010·Journal of Molecular and Cellular Cardiology·R John Solaro, Jolanda van der Velden

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.