Mar 1, 1978

Stuttering: high-level mistranslation in animal and bacterial cells

Proceedings of the National Academy of Sciences of the United States of America
J ParkerC P Stanners

Abstract

In both bacterial and mammalian cells, extreme starvation for certain amino acids resulted in translational errors that could be easily detected by two-dimensional polyacrylamide gel electrophoresis. On two-dimensional gels, the faulty proteins were shown as a trail of spots with molecular weights similar to those of the authentic proteins but separated in the isoelectric focusing dimension, a phenomenon we call "stuttering." The direction of charge shift depended on the amino acid substituted and could be predicted from misreading of pyrimidines for purines at the third position of the codon. It is expected that this phenomenon will provide a rapid means of measuring the fidelity of the translational machinery from cell type to cell type.

Mentioned in this Paper

Alkalescens-Dispar Group
Amino Acids, I.V. solution additive
Protein Biosynthesis
Sense Codon
Codon Genus
Codon (Nucleotide Sequence)
Isoelectric Focusing
Amino Acyl-tRNA Synthetases
Cell Type
Pyrimidines

About this Paper

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