Stycholysin II, a cytolysin from the sea anemone Stichodactyla helianthus promotes higher hemolysis in aged red blood cells

Toxicon : Official Journal of the International Society on Toxinology
Gloria CeledónM E Lanio

Abstract

We have investigated the relationship between the status of red blood cells (RBCs) and their susceptibility to toxin sticholysin II (StII) hemolytic activity; we have evaluated this effect in different RBC ensembles, comprising young and old cells, and in cells partially damaged by their pre-exposition to a free radical source. Upon action of StII, young cell populations are less prone to hemolysis than the whole population, while old cell populations and peroxyl-oxidized red cells are lysed faster than the whole population. Cell K(+) content was higher in young cells and lower in both senescent cells and in peroxyl-damaged cells relative to whole cell population. The relevance of cell K(+) content in St II-induced lysis was shown when external Na(+) was partially replaced by K(+); under this condition, RBC lysed faster in the presence of St II but no difference was observed among young cells, whole cells population and peroxyl-damaged cells; only old cells lysed faster that the whole population, response that can be due to an enhanced St II-induced pore formation as supported by evaluation of St II irreversible binding to RBC. It is concluded that this factor and the amount of intracellular K(+) are the dominant parameters tha...Continue Reading

References

Sep 10, 1985·Biochimica Et Biophysica Acta·M A Zanner, W R Galey
May 30, 1998·Toxicon : Official Journal of the International Society on Toxinology·C AlvarezE A Lissi
Sep 2, 1998·Toxicon : Official Journal of the International Society on Toxinology·I F PazosA M Campos
Aug 2, 2001·Toxicon : Official Journal of the International Society on Toxinology·D MartinezE Lissi
Feb 28, 2003·American Journal of Physiology. Cell Physiology·Isabel BizeCarlo Brugnara
Mar 22, 2003·Blood·Fausta Omodeo-SalèDonatella Taramelli
Apr 18, 2003·Cell Death and Differentiation·K S LangS M Huber
Mar 17, 2004·Toxicon : Official Journal of the International Society on Toxinology·María Cristina PicoAnselmo Otero
May 14, 2004·Annual Review of Biophysics and Biomolecular Structure·Kai Simons, Winchil L C Vaz
Jul 2, 2005·Toxicon : Official Journal of the International Society on Toxinology·G CeledonE Lissi
Nov 23, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Paolo AreseEvelin Schwarzer
Jan 31, 2006·The FEBS Journal·Jorge Alegre-CebolladaAlvaro Martínez del Pozo
Aug 1, 2006·Journal of Theoretical Biology·Piotr H PawlowskiPiotr Zielenkiewicz
Oct 28, 2006·Toxicon : Official Journal of the International Society on Toxinology·F PazosE Lissi
Nov 9, 2006·Free Radical Research·Suresh I S Rattan
Nov 23, 2006·Toxicon : Official Journal of the International Society on Toxinology·Diana MartínezEduardo Lissi

❮ Previous
Next ❯

Citations

Jul 1, 2009·Toxicon : Official Journal of the International Society on Toxinology·Augusto BellomioJuan Manuel González-Mañas
Mar 18, 2016·The Journal of Biological Chemistry·Monika Bokori-BrownC Peter Winlove
Jan 1, 2016·Fiziolohichnyĭ zhurnal·N G Zemlianskykh, L A Babijchuk

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.