SU-E-T-03: Justification and Feasibility of Neural Stem Cell Sparing in Whole Brain Irradiation Using VMAT

Medical Physics
T PhillipsLei Xing

Abstract

To determine the impact of radiation on neural stem cells and examine the feasibility of neural stem cell sparing using volumetric modulated arc therapy (VMAT) for whole brain irradiation. Murine neural stem cells were harvested from the sub-ventricular zone (SVZ), cultured and assessed for radio-sensitivity and differentiation potential in response to radiation (0-6Gy). Clonogenic survival of these cells was compared to that of tumor cells derived from the murine PTEN-/-/Kras+ spontaneous brain tumor model. The differentiation potential of neural stem cells surviving irradiation was examined by allowing the cells to differentiate for five days and analyzing cell types. In order to address the feasibility of neural stem cell sparing, a typical 3D whole brain treatment was compared to VMAT, in which the neural stem cell compartment, designated and contoured as the lateral ventricles with a 3-5mm margin, was spared. Whole brain dose coverage was maintained similarly to conventional 3D treatment, while minimizing the dose to the stem cell compartment. Neural stem cells are significantly more radio-sensitive than their tumor counterparts (2Gy, p=0.018), and the neuronal differentiation capacity of stem cells that survive radiation ...Continue Reading

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