Sub-cellular In-situ Characterization of Ferritin(iron) in a Rodent Model of Spinal Cord Injury

Scientific Reports
Angela R BlissettG Agarwal

Abstract

Iron (Fe) is an essential metal involved in a wide spectrum of physiological functions. Sub-cellular characterization of the size, composition, and distribution of ferritin(iron) can provide valuable information on iron storage and transport in health and disease. In this study we employ magnetic force microscopy (MFM), transmission electron microscopy (TEM), and electron energy loss spectroscopy (EELS) to characterize differences in ferritin(iron) distribution and composition across injured and non-injured tissues by employing a rodent model of spinal cord injury (SCI). Our biophysical and ultrastructural analyses provide novel insights into iron distribution which are not obtained by routine biochemical stains. In particular, ferritin(iron) rich lysosomes revealed increased heterogeneity in MFM signal from tissues of SCI animals. Ultrastructural analysis using TEM elucidated that both cytosolic and lysosomal ferritin(iron) density was increased in the injured (spinal cord) and non-injured (spleen) tissues of SCI as compared to naïve animals. In-situ EELs analysis revealed that ferritin(iron) was primarily in Fe3+ oxidation state in both naïve and SCI animal tissues. The insights provided by this study and the approaches utili...Continue Reading

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Citations

Oct 15, 2019·Nanoscale Advances·Joshua SiffordGunjan Agarwal
Dec 22, 2019·Experimental Neurology·Matthew T Goodus, Dana M McTigue
Apr 22, 2021·Metallomics : Integrated Biometal Science·Marina PlaysRaphaël Rodriguez
Dec 22, 2020·Journal of Magnetism and Magnetic Materials·Kevin J WalshGunjan Agarwal

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Methods Mentioned

BETA
X-ray
transmission electron microscopy
AFM

Software Mentioned

Image J
Nanoscope
NIH Image J

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