Sub-fertility in crossbred bulls: deciphering testicular level transcriptomic alterations between zebu (Bos indicus) and crossbred (Bos taurus x Bos indicus) bulls.

BMC Genomics
Kamaraj ElangoTirtha Kumar Datta

Abstract

The incidence of poor semen quality and sub-fertility/infertility is higher in crossbred as compared to Zebu males. Several attempts have been made to understand the possible reasons for higher incidence of fertility problems in crossbred males, at sperm phenotype, proteome and genome level but with variable results. Since the quality of the ejaculated spermatozoa is determined by the testicular environment, assessing the testicular transcriptome between these breeds would help in identifying the possible mechanisms associated with infertility in crossbred bulls. However, such information is not available. We performed global transcriptomic profiling of testicular tissue from crossbred and Zebu bulls using Agilent Bos taurus GXP 8X60k AMADID: 29411 array. To the best of our knowledge, this is the first study comparing the testicular mRNAs between crossbred and Zebu bulls. Out of the 14,419 transcripts detected in bovine testis, 1466 were differentially expressed between crossbred and Zebu bulls, in which 1038 were upregulated and 428 were downregulated in crossbred bulls. PI4KB and DPY19L2 genes, reported to be involved in sperm capacitation and acrosome formation respectively, were among the top 10 downregulated transcripts in...Continue Reading

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Citations

Oct 26, 2020·Molecular Biology Reports·Varinder Singh RainaVikas Vohra
May 28, 2021·Frontiers in Cell and Developmental Biology·Mani Arul PrakashTirtha Kumar Datta
Jul 27, 2021·Frontiers in Cell and Developmental Biology·Arumugam KumaresanJane M Morrell
Jul 3, 2021·Andrologia·Nilendu PaulArumugam Kumaresan

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Methods Mentioned

BETA
Chip
ubiquitination
dissection
in-vitro transcription
PCR
electrophoresis

Software Mentioned

SPSS
PANTHER
Cluego app
Cytoscape
DAVID
GeneSpring GX
DAVID Bioinformatics Resources
Feature Extraction

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