Sub-nanoliter, real-time flow monitoring in microfluidic chips using a portable device and smartphone

Scientific Reports
Yuksel Temiz, Emmanuel Delamarche

Abstract

The ever-increasing need for portable, easy-to-use, cost-effective, and connected point-of-care diagnostics (POCD) has been one of the main drivers of recent research on lab-on-a-chip (LoC) devices. A majority of these devices use microfluidics to manipulate precisely samples and reagents for bioanalysis. However, filling microfluidic devices with liquid can be prone to failure. For this reason, we have implemented a simple, yet efficient method for monitoring liquid displacement in microfluidic chips using capacitive sensing and a compact (75 mm × 30 mm × 10 mm), low-cost ($60), and battery-powered (10-hour autonomy) device communicating with a smartphone. We demonstrated the concept using a capillary-driven microfluidic chip comprising two equivalent flow paths, each with a total volume of 420 nL. Capacitance measurements from a pair of electrodes patterned longitudinally along the flow paths yielded 17 pL resolution in monitoring liquid displacement at a sampling rate of 1 data/s (~1 nL/min resolution in the flow rate). We characterized the system using human serum, biological buffers, and water, and implemented an algorithm to provide real-time information on flow conditions occurring in a microfluidic chip and interactive ...Continue Reading

References

Mar 10, 2004·Lab on a Chip·John Collins, Abraham P Lee
Jul 27, 2005·IEEE Transactions on Bio-medical Engineering·Wendy FranksAndreas Hierlemann
Nov 16, 2005·Lab on a Chip·Jian Wu, Jing Ye
Aug 13, 2008·Analytical and Bioanalytical Chemistry·Geertruida A Posthuma-TrumpieAart van Amerongen
May 14, 2011·Advanced Materials·Luc GervaisEmmanuel Delamarche
Apr 26, 2013·Chemical Society Reviews·Xiliang Luo, Jason J Davis
Apr 15, 2014·Advanced Materials·Hyojin KoKwanwoo Shin
Sep 18, 2014·Lab on a Chip·A AlfadhelJ Kosel
Mar 24, 2015·Biosensors & Bioelectronics·Cédric BathanyYoon-Kyoung Cho
Apr 17, 2015·Malaria Journal·UNKNOWN WHO Malaria Policy Advisory Committee and Secretariat
Sep 1, 2015·Biosensors & Bioelectronics·Josiane P LafleurJörg P Kutter
Jul 19, 2016·Lab on a Chip·Mitchell Boyd-MossKhashayar Khoshmanesh
Feb 15, 2017·Analytical Chemistry·Jinhong Guo
Mar 16, 2017·Lab on a Chip·Despina Moschou, Angeliki Tserepi
Jan 11, 2018·Scientific Reports·Mohammad Hossein ZarifiAmir Sanati-Nezhad

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Citations

Nov 23, 2019·Scientific Reports·Marie L SalvaEmmanuel Delamarche
Oct 3, 2020·Bioengineering & Translational Medicine·Xiaona WenRizia Bardhan
Feb 4, 2021·APL Bioengineering·Jose M AyusoDavid J Beebe
Mar 24, 2021·Scientific Reports·Jianye SuiMehdi Javanmard
Aug 5, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Emmanuel DelamarcheGovind V Kaigala
Oct 17, 2019·Analytical Chemistry·Chuchu Qin, Purnendu K Dasgupta
Sep 17, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Jing J LeeAshleigh B Theberge
Aug 14, 2020·Chemical Reviews·Gurpur Rakesh D Prabhu, Pawel L Urban

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Methods Mentioned

BETA
chip
chips

Software Mentioned

Adafruit
RealTerm
Arduino IDE
Windows
Wire
Arduino
ImageJ
GFX
Edit
Dimension Elite

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