(Sub)-picosecond spectral evolution of fluorescence in photoactive proteins studied with a synchroscan streak camera system.

Photochemistry and Photobiology
Ivo H M van StokkumJohn T M Kennis

Abstract

The spectral evolution of three photoactive proteins has been investigated by measuring the fluorescence with good temporal and wavelength resolution and a high signal-to-noise ratio. Upon excitation at 400 nm wild-type (wt) PYP both at neutral pH and in the low-pH blueshifted pBdark state exhibited a strong quenching of the fluorescence, the major part of which could be described by lifetimes of about 1.7 and 7.7 ps. The remaining fluorescence decay occurred multiexponentially with lifetimes between 30 and 125 ps. Additionally, in wtPYP at neutral pH, a dynamic Stokes shift was found to occur with a time constant of about 0.25 ps. In a PYP preparation that was reconstituted with the chromophore 7-hydroxy-coumarin-3- carboxylic acid rather than the native coumaric acid, and which is therefore not capable of performing the cis-trans-isomerization that initiates the photocycle in wtPYP, the fluorescence was found to decay multiexponentially with lifetimes of 51 ps, 0.33 and 3.77 ns. Additionally, dynamic Stokes shifts were observed with time constants of about 0.1 and 3.5 ps. Upon comparison of the dynamics of this preparation with that of wtPYP the multiexponential decay with lifetimes of 1.7 and 7.7 ps found in wtPYP was attrib...Continue Reading

References

May 1, 1982·Biophysical Journal·J F NagleR H Lozier
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M ChattorajS G Boxer
Jul 1, 1996·Biophysical Journal·M E Van BrederodeK J Hellingwerf
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·K C HassonP A Anfinrud
Oct 1, 1996·Nature Biotechnology·F YangG N Phillips
Oct 6, 1998·Annual Review of Biochemistry·R Y Tsien
Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Samir Kumar PalAhmed H Zewail
Jul 9, 2004·Biochimica Et Biophysica Acta·Ivo H M van StokkumRienk van Grondelle
Dec 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·John T M KennisRienk van Grondelle
Mar 3, 2005·Journal of the American Chemical Society·Deborah Stoner-MaPeter J Tonge

❮ Previous
Next ❯

Citations

Jul 2, 2009·Photosynthesis Research·Ulai Noomnarm, Robert M Clegg
Oct 7, 2011·Journal of the American Chemical Society·Lijin TianHerbert van Amerongen
Dec 22, 2010·The Journal of Physical Chemistry. a·Olga A SytinaMarie Louise Groot
Mar 29, 2013·The Journal of Physical Chemistry. B·John T M KennisRebekka M Wachter
Aug 19, 2011·Physical Chemistry Chemical Physics : PCCP·Mariangela Di DonatoMarie Louise Groot
Apr 5, 2012·Physical Chemistry Chemical Physics : PCCP·Bart van OortIvo H M van Stokkum
Oct 24, 2012·Physical Chemistry Chemical Physics : PCCP·Craig N LincolnJasper J van Thor
Feb 11, 2014·Interface Focus·John T M Kennis, Tilo Mathes
Jan 26, 2011·Photochemistry and Photobiology·Ivo H M van StokkumJohn T M Kennis
Oct 30, 2016·Biochimica Et Biophysica Acta. Bioenergetics·Joris J SnellenburgIvo H M van Stokkum
May 9, 2015·Physical Chemistry Chemical Physics : PCCP·Marc Nadal-FerretJosé M Lluch
May 26, 2007·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Erwin LangJürgen Köhler
Apr 4, 2018·Nature Plants·Shazia FarooqHerbert van Amerongen
Oct 19, 2010·The Journal of Physical Chemistry Letters·Elizabeth C CarrollDelmar S Larsen
Dec 12, 2018·Journal of the American Chemical Society·María Del Carmen MarínMassimo Olivucci

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence spectroscopy
chip

Software Mentioned

EAS
SAS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
Delmar S Larsen, Rienk van Grondelle
The Journal of Physical Chemistry. B
Janne A IhalainenRienk van Grondelle
Journal of Photochemistry and Photobiology. B, Biology
Roberta Croce, Herbert van Amerongen
© 2022 Meta ULC. All rights reserved