Sub-proteomic study on macrophage response to Candida albicans unravels new proteins involved in the host defense against the fungus

Journal of Proteomics
Jose Antonio Reales-CalderónConcha Gil

Abstract

In previous proteomic studies on the response of murine macrophages against Candida albicans, many differentially expressed proteins involved in processes like inflammation, cytoskeletal rearrangement, stress response and metabolism were identified. In order to look for proteins important for the macrophage response, but in a lower concentration in the cell, 3 sub-cellular extracts were analyzed: cytosol, organelle/membrane and nucleus enriched fractions from RAW 264.7 macrophages exposed or not to C. albicans SC5314 for 3 h. The samples were studied using DIGE technology, and 17 new differentially expressed proteins were identified. This sub-cellular fractionation permitted the identification of 2 mitochondrion proteins, a membrane receptor, Galectin-3, and some ER related proteins, that are not easily detected in total cell extracts. Besides, the study of different fractions allowed us to detect, not only total increase in Galectin-3 protein amount, but its distinct allocation along the interaction. The identified proteins are involved in the pro-inflammatory and oxidative responses, immune response, unfolded protein response and apoptosis. Some of these processes increase the host response and others could be the effect of C...Continue Reading

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Citations

Jan 5, 2014·Molecular and Cellular Biochemistry·Parveen PahujaMunish Puri
Feb 26, 2016·Frontiers in Microbiology·S P SmeekensM G Netea
May 17, 2015·Journal of Proteomics·Noemí BujánBeatriz Magariños
Jul 19, 2015·Microbial Pathogenesis·Mayra Denisse Ramírez-QuijasMayra Cuéllar-Cruz
Oct 16, 2016·Journal of Proteome Research·Jose Antonio Reales-CalderónConcha Gil
Oct 16, 2016·Mycopathologia·Fabio Seiti Yamada YoshikawaSandro Rogerio de Almeida

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