Sub-second 2D NMR spectroscopy at sub-millimolar concentrations

Journal of the American Chemical Society
Boaz ShapiraLucio Frydman

Abstract

A recently proposed protocol enables the acquisition of two-dimensional nuclear magnetic resonance (2D NMR) spectra within a single scan. A promising application opened up by this new data acquisition mode concerns its combination with active nuclear polarization methods, whereby spectroscopy is carried out on analytes whose spin magnetizations have been significantly enhanced over their Boltzmann thermal values. The present paper explores the potential of such combination, with the acquisition of peptide and protein 2D NMR 1H correlation spectra recorded after the samples had been subject to laser-driven chemically induced dynamic nuclear polarization (CIDNP). It is demonstrated that the speed and sensitivity enhancement afforded by these combined processes enables the acquisition of quality 2D NMR data sets within a fraction of a second, at analyte concentrations that are under 1 mM.

References

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Jul 26, 2002·Journal of the American Chemical Society·Nikolaus M LoeningRobert G Griffin
Aug 8, 2002·Journal of Magnetic Resonance·Keyang Ding, Angela M Gronenborn
Dec 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lucio FrydmanAdonis Lupulescu
Jan 30, 2003·Journal of the American Chemical Society·Seho Kim, Thomas Szyperski
Aug 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jan H Ardenkjaer-LarsenKlaes Golman
Sep 10, 2003·Journal of Biomolecular NMR·Ray Freeman, Eriks Kupce
Jan 20, 2004·Journal of Magnetic Resonance·Boaz ShapiraLucio Frydman
Sep 17, 2004·Journal of the American Chemical Society·Lucio FrydmanTali Scherf
Oct 14, 2005·Journal of the American Chemical Society·Yoav Shrot, Lucio Frydman

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Citations

May 10, 2011·Journal of the American Chemical Society·Jung Ho LeeSilvia Cavagnero
May 26, 2009·The Journal of Physical Chemistry. B·Ashok Sekhar, Silvia Cavagnero
Nov 13, 2008·Annual Review of Physical Chemistry·Mor Mishkovsky, Lucio Frydman
Jun 24, 2014·The Journal of Chemical Physics·Abhishek Banerjee, N Chandrakumar
Dec 6, 2006·The Journal of Chemical Physics·Yoav Shrot, Lucio Frydman
Oct 18, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Olga B Morozova, Konstantin L Ivanov
Mar 19, 2014·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Susanna Ahola, Ville-Veikko Telkki
Dec 11, 2017·Journal of Magnetic Resonance·Yoshitaka IshiiTakayuki Kamihara

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