Sub-second calcium coupling between outside medium and subplasmalemmal stores during overstimulation/depolarisation-induced ciliary beat reversal in Paramecium cells

Cell Calcium
H PlattnerJ Hentschel

Abstract

As amply documented by electrophysiology, depolarisation in Paramecium induces a Ca(2+) influx selectively via ciliary voltage-dependent Ca(2+)-channels, thus inducing ciliary beat reversal. Subsequent downregulation of ciliary Ca(2+) has remained enigmatic. We now analysed this aspect, eventually under overstimulation conditions, by quenched-flow/cryofixation, combined with electron microscope X-ray microanalysis which registers total calcium concentrations, [Ca]. This allows to follow Ca-signals within a time period (> or =30ms) smaller than one ciliary beat ( approximately 50ms) and beyond. Particularly under overstimulation conditions ( approximately 10(-5)M Ca(2+) before, 0.5mM Ca(2+) during stimulation) we find in cilia a [Ca] peak at approximately 80ms and its decay to near-basal levels within 110ms (90%) to 170ms (100% decay). This [Ca] wave is followed, with little delay, by a [Ca] wave into subplasmalemmal Ca-stores (alveolar sacs), culminating at approximately 100ms, with a decay to original levels within 170ms. Also with little delay [Ca] slightly increases in the cytoplasm below. This implies rapid dissipation of Ca(2+) through the ciliary basis, paralleled by a rapid, transient uptake by, and release from cortical...Continue Reading

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Citations

Sep 2, 2006·Annual Review of Physiology·Matthias Salathe
Jan 9, 2008·Cell Calcium·Yoshiaki Iwadate, Yasuo Nakaoka
Nov 30, 2012·The Journal of Eukaryotic Microbiology·Heidi K GrønlienOlav Sand
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Jul 22, 2014·FEMS Microbiology Ecology·Michael L EchevarriaAlison R Taylor
Dec 30, 2008·The Journal of Experimental Biology·Yasuo NakaokaNoboru Hashimoto

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