Subarachnoid injections of lysed blood induce the hsp70 stress gene and produce DNA fragmentation in focal areas of the rat brain

Stroke; a Journal of Cerebral Circulation
P G MatzF R Sharp

Abstract

Most experimental studies of subarachnoid hemorrhage have demonstrated little histological evidence of injury. In the present study we examined both the expression of the hsp70 heat-shock gene, a molecular marker of reversible neuronal injury, and DNA fragmentation, a marker of irreversible cell injury and death. Lysed blood, whole blood, oxyhemoglobin, bovine serum albumin, and saline were injected into the cisterna magna of adult rats. The induction of hsp70 mRNA and HSP70 heat-shock protein was assessed with the use of in situ hybridization and immunocytochemistry, respectively. Fragmentation of genomic DNA was studied by DNA nick end- labeling with the use of terminal deoxynucleotidyl transferase and biotinylated dATP. Expression of the hsp70 gene was not induced in the brains of rats injected with whole blood, oxyhemoglobin, bovine serum albumin, or saline. Lysed blood injections, however, induced hsp70 mRNA at 6 and 24 hours in the cerebellar hemispheres and in focal regions of the basal forebrain. HSP70 protein was induced by 24 hours and persisted for at least 4 days in the same regions. HSP70 protein was localized to patches of glial cells and occasional neurons in the forebrain. In the cerebellum HSP70 was localized t...Continue Reading

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Citations

Jun 26, 2003·Neurosurgery·Aaron S DumontKevin S Lee
Mar 28, 2013·Stroke Research and Treatment·Mohammed SabriR Loch Macdonald
Jan 1, 1997·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·D J ColeP M Patel
Feb 10, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Joseph F Clark, Frank R Sharp
Feb 3, 2009·The Journal of Surgical Research·Chih-Lung LinShen-Long Howng
Jun 9, 2006·Neurological Research·Fatima A Sehba, Joshua B Bederson
Feb 27, 1999·Renal Failure·T J BurkeN F Voelkel
Feb 11, 2000·Toxicologic Pathology·S Rajdev, F R Sharp
Mar 14, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·C P TurnerF R Sharp
Dec 21, 2018·Stroke; a Journal of Cerebral Circulation·Han-Gil JeongSeung-Hoon Lee
Jul 6, 2019·Journal of Cellular and Molecular Medicine·Anwen ShaoYongchuan Deng
Jul 14, 2000·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·C BeaulieuM E Moseley
Mar 17, 2000·Neurosurgery·H H Dietrich, R G Dacey

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