PMID: 8962161Dec 10, 1996Paper

Subcellular colocalization of the cellular and scrapie prion proteins in caveolae-like membranous domains

Proceedings of the National Academy of Sciences of the United States of America
M VeyS B Prusiner

Abstract

Results of transgenetic studies argue that the scrapie isoform of the prion protein (PrPSc) interacts with the substrate cellular PrP (PrPC) during conversion into nascent PrPSc. While PrPSc appears to accumulate primarily in lysosomes, caveolae-like domains (CLDs) have been suggested to be the site where PrPC is converted into PrPSc. We report herein that CLDs isolated from scrapie-infected neuroblastoma (ScN2a) cells contain PrPC and PrPSc. After lysis of ScN2a cells in ice-cold Triton X-100, both PrP isoforms and an N-terminally truncated form of PrPC (PrPC-II) were found concentrated in detergent-insoluble complexes resembling CLDs that were isolated by flotation in sucrose gradients. Similar results were obtained when CLDs were purified from plasma membranes by sonication and gradient centrifugation; with this procedure no detergents are used, which minimizes artifacts that might arise from redistribution of proteins among subcellular fractions. The caveolar markers ganglioside GM1 and H-ras were found concentrated in the CLD fractions. When plasma membrane proteins were labeled with the impermeant reagent sulfo-N-hydroxysuccinimide-biotin, both PrPC and PrPSc were found biotinylated in CLD fractions. Similar results on th...Continue Reading

References

Oct 1, 1992·Protein Science : a Publication of the Protein Society·K M PanS B Prusiner
Jan 1, 1992·Cold Spring Harbor Symposia on Quantitative Biology·Y S YingK G Rothberg
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J M GabrielS B Prusiner
Jun 1, 1990·The Journal of Cell Biology·A TaraboulosS B Prusiner
Oct 1, 1989·Archives of Biochemistry and Biophysics·T HaraguchiA Burlingame
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M P LisantiE Rodriguez-Boulan
Aug 13, 1985·Biochemistry·A L TarentinoT H Plummer
Dec 21, 1982·Biochemistry·S B PrusinerM P McKinley
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·E J SmartR G Anderson
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·K KanekoS B Prusiner
Nov 1, 1995·The Journal of Cell Biology·E J SmartR G Anderson
Jan 1, 1995·Brain Pathology·G A Wells, J W Wilesmith
Jan 1, 1994·Annual Review of Microbiology·S B Prusiner
Jan 27, 1995·Journal of Molecular Biology·H M SchätzlS B Prusiner
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S B PrusinerS J DeArmond
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K M PanF E Cohen
Jun 1, 1994·The Journal of Cell Biology·S L ShyngD A Harris
Aug 11, 1994·Nature·D A KociskoB Caughey
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R G Anderson
Feb 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R G Parton
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M RogersS B Prusiner
Jan 19, 1996·The Journal of Biological Chemistry·S Lehmann, D A Harris
May 17, 1996·The Journal of Biological Chemistry·C MineoR G Anderson

❮ Previous
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Citations

Jun 28, 2000·Microscopy Research and Technique·J G FournierV Grigoriev
Aug 24, 2006·Cellular and Molecular Life Sciences : CMLS·K Abid, C Soto
Oct 14, 2009·Cellular and Molecular Life Sciences : CMLS·Sabine GilchHermann M Schätzl
Dec 8, 2007·European Biophysics Journal : EBJ·Laura J VellaAndrew F Hill
Jan 5, 2008·Pflügers Archiv : European journal of physiology·Susanne WegmannDaniel J Muller
Mar 10, 2010·Apoptosis : an International Journal on Programmed Cell Death·Valentina CecariniAnna Maria Eleuteri
Jan 28, 2005·Cellular and Molecular Neurobiology·Jun-ichi Satoh, Takashi Yamamura
Sep 11, 2008·Glycoconjugate Journal·Alessandro PrinettiSandro Sonnino
Dec 4, 2003·Brain Research·Nuha HijaziRuth Gabizon
Nov 16, 2005·Comptes rendus biologies·Mari L DeMarco, Valerie Daggett
Apr 19, 2005·Journal of Neuroimmunology·Zehavit Kariv-InbalRuth Gabizon
May 22, 2003·Journal of Molecular Biology·Chongsuk RyouGiuseppe Legname
Jul 4, 1998·Brain Research. Brain Research Reviews·T HökfeltJ H Walsh
May 14, 2003·Molecular and Biochemical Parasitology·Jun WangGeorge A M Cross
Dec 26, 2001·Brain Research. Molecular Brain Research·P OstlundK Bedecs
Sep 12, 2001·Advanced Drug Delivery Reviews·L C Norkin
Aug 15, 2002·Neurochemistry International·Vilma Regina Martins, Ricardo Renzo Brentani
Nov 5, 1997·Biochimica Et Biophysica Acta·O NosjeanB Roux
May 19, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·S ThellungG Schettini
Apr 30, 1999·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·S LehmannA Mangé
Oct 1, 1998·Cellular Signalling·A SchlegelM P Lisanti
Jun 19, 2002·Trends in Biochemical Sciences·David Westaway, George A Carlson
Apr 3, 1999·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·R RiegerS Weiss
Apr 21, 2011·Nature Communications·R GooldS J Tabrizi
Dec 13, 1997·Nature Medicine·V R MartinsR R Brentani
Nov 4, 2000·The Biochemical Journal·F MeggioL A Pinna
Sep 28, 1998·The European Journal of Neuroscience·N SalèsK L Moya
Jan 5, 2000·Journal of Neurochemistry·O MilhavetS Lehmann
Jul 2, 2002·Journal of Neurochemistry·Yuval ShakedRuth Gabizon
Apr 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yervand Eduard KarapetyanCorinne Ida Lasmézas
Sep 15, 2009·The Journal of Biological Chemistry·Christian BachIna Vorberg
Jul 1, 2009·The Journal of Biological Chemistry·David R TaylorNigel M Hooper
Apr 2, 2010·The Journal of Biological Chemistry·Christopher F HarrisonAndrew F Hill
Apr 30, 2010·The Journal of Biological Chemistry·Clive BateAlun Williams
Jan 21, 2012·The Journal of Biological Chemistry·Clive Bate, Alun Williams

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