PMID: 7372714May 1, 1980Paper

Subcellular compartmentalization of saccharide moieties in cultured normal and malignant cells

The Journal of Cell Biology
I VirtanenP Laurila

Abstract

We studied subcellular localization of saccharide moieties in cultured normal and malignant cells fixed in paraformaldehyde and treated with a nonionic detergent, using lectins specific for various surgar residues as probes in fluorescence microscopy. In normal cells, concanavalin A and Lens culinaris agglutinin, specific for mannose-rich carbohydrate cores in glycoproteins, labeled the endoplasmic reticulum as a wide perinuclear region. Other lectins, on the other hand, stained the Golgi apparatus as a juxtanuclear reticular structure. A similar compartmentalization was also seen in all malignant cells studied, although the Golgi apparatus in these cells was distinctly vesicular in appearance. Our results indicate that saccharide moieties in both normal and malignant cells are similarly compartmentalized, and thus speak in favor of a unidirectional subcellular flow for both membrane and secreted glycoconjugates.

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Citations

Aug 1, 1993·Journal of Cellular Biochemistry·N McFarlane-AndersonN Bégin-Heick
Jan 1, 1986·Archives of Virology·T KohamaA Sugiura
Feb 1, 1996·Histochemistry and Cell Biology·G HerrmanK Spanel-Borowski
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Aug 22, 2007·Journal of Biomedical Science·Xiwen XiongXiang Gao
Jul 28, 1999·Bone·D J DallasT M Skerry
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·N G Lipsky, R E Pagano
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S L Zebedee, R A Lamb
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·O C MartinR E Pagano
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·P A FernandezJ Yuan
Feb 3, 2009·The Journal of Biological Chemistry·Yasuhiko KizukaShogo Oka
Oct 1, 1985·The Journal of Cell Biology·L J RizzoloD D Sabatini
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Jun 1, 1990·The Journal of Cell Biology·A TaraboulosS B Prusiner
Dec 1, 1990·The Journal of Cell Biology·P A BresnahanG Thomas
Dec 1, 1990·The Journal of Cell Biology·T David-Pfeuty, Y Nouvian-Dooghe
Apr 1, 1992·The Journal of Cell Biology·T A Woodford-ThomasJ E Dixon
Sep 1, 1992·The Journal of Cell Biology·I ToyoshimaM P Sheetz

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