Subcellular Compartmentation of Alternatively Spliced Transcripts Defines SERINE/ARGININE-RICH PROTEIN30 Expression

Plant Physiology
Lisa HartmannAndreas Wachter

Abstract

Alternative splicing (AS) is prevalent in higher eukaryotes, and generation of different AS variants is tightly regulated. Widespread AS occurs in response to altered light conditions and plays a critical role in seedling photomorphogenesis, but despite its frequency and effect on plant development, the functional role of AS remains unknown for most splicing variants. Here, we characterized the light-dependent AS variants of the gene encoding the splicing regulator Ser/Arg-rich protein SR30 in Arabidopsis (Arabidopsis thaliana). We demonstrated that the splicing variant SR30.2, which is predominantly produced in darkness, is enriched within the nucleus and strongly depleted from ribosomes. Light-induced AS from a downstream 3' splice site gives rise to SR30.1, which is exported to the cytosol and translated, coinciding with SR30 protein accumulation upon seedling illumination. Constitutive expression of SR30.1 and SR30.2 fused to fluorescent proteins revealed their identical subcellular localization in the nucleoplasm and nuclear speckles. Furthermore, expression of either variant shifted splicing of a genomic SR30 reporter toward SR30.2, suggesting that an autoregulatory feedback loop affects SR30 splicing. We provide evidence...Continue Reading

Citations

May 22, 2019·Plant & Cell Physiology·Misato Ohtani, Andreas Wachter
Aug 31, 2018·Frontiers in Plant Science·Dóra Szakonyi, Paula Duque
Feb 23, 2019·Nucleic Acids Research·Ibtissam JabreNaeem H Syed
Feb 11, 2020·Frontiers in Plant Science·Candida NibauNicola Cavallari
Jun 28, 2019·Frontiers in Plant Science·Saurabh ChaudharyNaeem H Syed
Apr 16, 2021·Frontiers in Plant Science·Remus R E RosenkranzSotirios Fragkostefanakis

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