PMID: 9421146Jan 8, 1998Paper

Subcellular concentration of beta-dystroglycan in photoreceptors and glial cells of the chick retina

The Journal of Comparative Neurology
M BlankS Kröger

Abstract

Mutations in the dystrophin-glycoprotein complex cause muscle degeneration and dysfunctions in the central nervous system, including an impaired synaptic transmission in the outer plexiform layer (OPL) of the retina. To investigate the basis for this ocular phenotype, we analyzed the distribution of beta-dystroglycan, a central member of the dystrophin-glycoprotein complex, in the chick retina by using the 43DAG/8D5 monoclonal antibody. This antibody reacted specifically with chick beta-dystroglycan, as indicated by its staining of the neuromuscular junction, and its reactivity with a single 43-kilodalton band in Western blots. In the retina, beta-dystroglycan was highly concentrated in the OPL and at the vitreal border of the retina, around the inner limiting membrane. Mechanically isolated and flat-mounted inner limiting membranes were stained by the anti-beta-dystroglycan antibody, and this immunoreactivity could be extracted by detergent, indicating that beta-dystroglycan is associated with membranous structures bound to the basal lamina. Consistently, electron microscopy showed a concentration of beta-dystroglycan in the endfeet of Müller glial cells exclusively in the region of direct contact to the inner limiting membran...Continue Reading

Citations

Oct 23, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jakob S SatzKevin P Campbell
Nov 10, 2009·Molecular Neurobiology·Gonneke S K PilgramJasprina N Noordermeer
Aug 5, 2010·The Journal of Biological Chemistry·Motoi KanagawaTatsushi Toda

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