Subcellular distribution and phosphorylation of the nuclear localization signal binding protein, NBP60

Experimental Cell Research
S KawahireT Horigome

Abstract

We previously purified a nuclear localization signal binding protein, NBP60, from rat liver (1993, J. Biochem. 113, 308-313). In this study, the subcellular localization of NBP60 was examined using anti-NBP60. Most NBP60 was found to be localized in the nuclear envelope fraction of rat liver obtained on cell fractionation followed by immunoblotting. Staining of the nuclei of cultured cells by the antibody was observed on immunofluorescence microscopy. NBP60 was widely detected in rat nuclear fractions prepared from other tissues and also in nuclei of cultured cells derived from other species. It was shown by immunoelectron microscopy that most NBP60 is present in the nuclear envelope and at least some of that is present on nuclear pore complexes. Although NBP60 was localized in the nuclear envelope in interphase cells, it diffused into the cytoplasm in the mitotic phase. The purified NBP60 was highly phosphorylated by a cdc2 mitotic kinase, whereas nuclear pore proteins p144, p62, p60, and p54 were not phosphorylated by the kinase directly. NBP60 was also phosphorylated by protein kinase A, calmodulin-dependent protein kinase II, and casein kinase II. The phosphorylation of NBP60 by cdc2 kinase and/or the other kinases may be r...Continue Reading

Citations

Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·T D Sweitzer, J A Hanover
Jun 22, 1999·Archives of Biochemistry and Biophysics·M W MillerJ A Hanover
Feb 16, 2002·European Journal of Biochemistry·Makoto TakanoTsuneyoshi Horigome
Jan 4, 2008·Pflügers Archiv : European journal of physiology·Yasuhiro HiranoKunio Takeyasu
Jan 14, 2004·The Journal of Biological Chemistry·Makoto TakanoTsuneyoshi Horigome
Aug 8, 2001·Journal of Neuroscience Methods·J BergquistR Ekman

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