Sep 24, 1976

Subcellular distribution and properties of guanylate cyclase in rat cerebellum

Biochimica Et Biophysica Acta
K NakazawaT Saito


In rat cerebellum the major portion of guanylate cyclase was found to be particulate-bound. The properties of particulate and supernatant guanylate cyclases from the cerebellum were comparatively examined. Both enzymes required the same optimal concentration of Mn2+ and were stimulated by Ca2+ in the presence of a low concentration of Mn2+. But dispersion of the particulate enzyme with Triton X-100 altered the Mn2+ concentration producing maximum activity and the inhibitory effect of Ca2+. The subcellular distributions of guanylate and adenylate cyclases were also studied in rat cerebellum. The major portions of the two cyclases were found in the mitochondrial fraction. The submitochondrial fractions separated by sucrose gradient showed that the major activities of both cyclases were concentrated in the fraction containing mainly nerve ending particles.

Mentioned in this Paper

Enzymes, antithrombotic
Rat Cerebellum
Nerve Endings
Enzymes for Treatment of Wounds and Ulcers
Cortical Cell Layer of the Cerebellum

About this Paper

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