PMID: 2504884Oct 1, 1989Paper

Subcellular distribution of 65,000 calmodulin-binding protein (p65) and synaptophysin (p38) in adrenal medulla

Journal of Neurochemistry
S FournierJ M Trifaró

Abstract

Both neuronal and endocrine cells contain secretory vesicles that store and release neurotransmitters and peptides. Neuronal cells release their secretory material from both small synaptic vesicles and large dense-core vesicles (LDCVs), whereas endocrine cells release secretory products from LDCVs. Neuronal small synaptic vesicles are known to express three integral membrane proteins: 65,000 calmodulin-binding protein (65-CMBP) (p65), synaptophysin (p38), and SV2. A controversial question surrounding these three proteins is whether they are present in LDCV membranes of endocrine and neuronal cells. Sucrose density centrifugation of adrenal medulla was performed to study and compare the subcellular distribution of two of these small synaptic vesicle proteins (65-CMBP and synaptophysin). Subsequent immunoblotting and 125I-Protein A binding experiments performed on the fractions obtained from sucrose gradients showed that 65-CMBP was present in fractions corresponding to granule membranes and intact chromaffin granules. Similar immunoblotting and 125I-Protein A binding experiments with synaptophysin antibodies showed that this protein was also present in intact granules and granule membrane fractions. However, an additional membra...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 14, 1976·Biochimica Et Biophysica Acta·J M Trifaró, A C Duerr
Oct 4, 1985·Science·R B Kelly
Jan 1, 1987·Annals of the New York Academy of Sciences·P de Camilli, F Navone
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D T PangP Greengard
Nov 1, 1988·Journal of Neurochemistry·S Fournier, J M Trifaró
Jan 1, 1987·Annals of the New York Academy of Sciences·J M Trifaró, S Fournier
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R JahnP Greengard
May 20, 1981·Biochimica Et Biophysica Acta·J A van der MeulenS Grinstein

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Citations

Feb 13, 1992·European Journal of Pharmacology·J M TrifaróA Rodríguez Del Castillo
Feb 1, 1996·Micron : the International Research and Review Journal for Microscopy·G K Zupanc
Dec 14, 1996·Journal of the Autonomic Nervous System·X E Hou, A Dahlström
Nov 20, 2002·Annals of the New York Academy of Sciences·Sergio D RoséJosé-María Trifaró
Jan 16, 2007·The Journal of Urology·Richard A AshleyJohn C Cheville
Apr 5, 2003·Journal of Neurochemistry·Sergio D RoséJosé-María Trifaró
Oct 7, 1997·FEBS Letters·R D Burgoyne, G Williams
Aug 1, 1990·FEBS Letters·H Zimmermann
Dec 27, 1990·European Journal of Biochemistry·M BählerM Da Prada

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