Subcellular distribution of cadmium and its cellular ligands in mussel digestive gland cells as revealed by combined autometallography and X-ray microprobe analysis

The Histochemical Journal
Manu SotoIonan Marigómez

Abstract

Autometallography (AMG) and electron probe X-ray microanalysis (EPXMA) were applied in combination to determine the subcellular distribution of Cd and its subcellular ligands in the digestive gland cells of Cd-exposed mussels Mytilus galloprovincialis. Black silver deposits (BSD), which reveal the presence of metals when AMG is applied, were only localized in digestive cell lysosomes. Digestive cell cytoplasm and basophilic cells were devoid of BSD. EPXMA (static probe and X-ray mapping) indicated that Cd, S (possibly associated with metallothioneins or metallothionein-like proteins) and autometallographical Ag ions are co-localized within digestive cell lysosomes. In addition, Cd and S co-occur in the absence of Ag in the cytosol of digestive cells. AMG does not reveal the presence of the Cd 'pool' strongly bound to cytosolic Cd-metallothionein complexes; only 'free' Cd or Cd supposedly loosely bound to (semi)digested metallothionein within lysosomes was revealed. The levels of lysosomal Cd were indirectly quantified by stereology as the volume density of BSD (V(v)BSD). Significantly higher values were recorded in Cd-exposed mussels compared with controls at all exposure times. However, V(v)BSD values were lower at days 7 and ...Continue Reading

Citations

Jun 28, 2005·Archives of Environmental Contamination and Toxicology·A J Morgan, M P Turner
Oct 26, 2018·Environmental Science and Pollution Research International·Melina AbdouManu Soto
Aug 19, 2018·Histochemistry and Cell Biology·Nicole M PirozziBen N G Giepmans
Apr 24, 2012·The Science of the Total Environment·Steven BrooksIonan Marigómez
Jul 9, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Kaida XuPengfei Li
Aug 21, 2013·ACS Chemical Biology·Malgorzata KorbasGraham N George
Jan 29, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Urtzi IzagirreIonan Marigómez

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