PMID: 7452275Sep 1, 1980

Subcellular distribution of glutamate decarboxylase in rat olfactory bulb: high content in dendrodendritic synaptosomes

Journal of Neurochemistry
M R Quinn, R H Cagan

Abstract

The olfactory bulbs in the CNS contain reciprocal dendrodendritic synapses between the granule cells and the secondary dendrites of mitral cells. Based on pharmacologic and electrophysiologic evidence, these synapses are believed to utilize GABA as an inhibitory neurotransmitter. A dendrodendritic synaptosomal fraction has been isolated from rat olfactory bulbs. The upper portion (PB) of the crude nuclear pellet contains 30-40% of the GAD (glutamate decarboxylase) activity of the olfactory bulb homogenate. When PB is purified on a discontinuous sucrose density gradient, 78-85% of the GAD activity is localized to the region containing the dendrodendritic synaptosomes, which were identified by transmission electron microscopy. The presence of a substantial proportion of GAD, the enzyme that catalyzes synthesis of GABA, in the DDS provides neurochemical support for the hypothesis that GABA functions at the reciprocal dendrodendritic synapses in the olfactory bulb.

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Citations

Mar 1, 1991·Neurochemical Research·M G Erlander, A J Tobin
Jan 1, 1990·Brain Research. Developmental Brain Research·D MangouraA Vernadakis
Dec 18, 1992·Brain Research. Developmental Brain Research·S KentrotiA Vernadakis
Jan 1, 1991·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C Brodie, A Vernadakis
Jan 1, 1991·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C Brodie, A Vernadakis
Oct 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·P E SpoerriA Vernadakis
Apr 1, 1984·Neurochemical Research·S C ChengI A Minieka
Feb 1, 1988·The International Journal of Neuroscience·J A Jesberger, J S Richardson
Jul 1, 1991·Neuron·M G ErlanderA J Tobin

Related Concepts

Carboxy-Lyases
Cell Fractionation
Centrifugation
Dendrites
Glutamate Decarboxylase
Electron Microscopy
Accessory Olfactory Bulb
Synaptosomes

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