PMID: 9174163May 1, 1997Paper

Subcellular distribution of Xenopus XEL-1 protein, a member of the neuron-specific ELAV/Hu family, revealed by epitope tagging

DNA and Cell Biology
M PerronL Théodore

Abstract

Drosophila and vertebrate elav/Hu genes are involved in the development and the maintenance of the nervous system. They all encode proteins that contain three RNA recognition motifs (RRM) and are thus expected to play a role in RNA metabolism. Drosophila ELAV and RBP9 proteins were reported to be exclusively distributed in nuclei of neurons, whereas known human Hu proteins display a bipartite nuclear and cytoplasmic distribution. We have previously isolated a member of this family in Xenopus, Xel-1, that is exclusively expressed in neural tissues from the early tailbud stage onward. In the present study, we report on the subcellular distribution of XEL-1 protein using myc epitope tagging, a strategy allowing the study of a single member of the ELAV/Hu family. We show that the subcellular distribution of exogenous XEL-1 protein in neural tissues depends on developmental stages. In the neural tube at the neurula stage, where endogenous Xel-1 is not expressed, exogenous tagged XEL-1 protein is localized in both the nucleus and the cytoplasm. At the tailbud stage, where endogenous Xel-1 is expressed, exogenous tagged XEL-1 protein is localized essentially in the cytoplasm of neural tube cells. In contrast, exogenous Drosophila ELAV...Continue Reading

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Citations

Sep 5, 1998·Brain Research. Developmental Brain Research·G H ClaytonG C Owens
Feb 3, 1999·Annual Review of Genetics·J W Jarvik, C A Telmer
Dec 4, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Reginald Van WayenberghEric J Bellefroid
Jun 21, 2002·Developmental Biology·Wendy V GerberPaul A Krieg
Jan 1, 2003·The Journal of Comparative Neurology·Akiko M Vankirk, Christine A Byrd

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