PMID: 11906903Mar 22, 2002Paper

Subcellular distribution of ZP1, ZP2, and ZP3 glycoproteins during folliculogenesis and demonstration of their topographical disposition within the zona matrix of mouse ovarian oocytes

Biology of Reproduction
Majid El-MestrahFrederick W K Kan

Abstract

The zona pellucida (ZP) is an extracellular coat synthesized and secreted by the oocyte during follicular development and surrounding the plasma membrane of mammalian eggs. To date, the mechanism of synthesis and secretion, mode of assembly, and intracellular trafficking of the ZP glycoproteins have not been fully elucidated. Using antibodies against mouse ZP1, ZP2, and ZP3 in conjunction with the protein A-gold technique, we have shown an association of immunolabeling with the Golgi apparatus, secretory granules, and a complex structure called vesicular aggregate, respectively, in mouse ovarian follicles. In contrast, the neighboring granulosa cells were not reactive to any of the three antibodies used. Immunolabeling of ZP1, ZP2, and ZP3 was detected throughout the entire thickness of the ZP, irrespective of the developmental stage of ovarian follicles. Double and triple immunolocalization studies, using antibodies tagged directly to different sizes of gold particles, revealed an asymmetric spatial distribution of the three ZP glycoproteins in the zona matrix at various stages of follicular development. All three glycoproteins were specifically localized over small patches of darkly stained flocculent substance dispersed thro...Continue Reading

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Citations

Oct 19, 2006·Molecular and Cellular Biology·Tanya HoodbhoyJurrien Dean
Dec 17, 2003·Reproductive Biology and Endocrinology : RB&E·Jacques E FléchonJan Motlik
Mar 8, 2011·Journal of Cell Science·Maria Jimenez-Movilla, Jurrien Dean
Mar 27, 2007·Okajimas Folia Anatomica Japonica·Yutaka KohataFumioki Yasuzumi
Jun 12, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Hongbin XuNongnuj Tanphaichitr
Nov 16, 2010·Theriogenology·M J Izquierdo-RicoM Avilés
Feb 6, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yeying SunChunmei Li
Oct 16, 2014·Zygote : the Biology of Gametes and Early Embryos·Bartosz KempistyMaciej Zabel
May 17, 2006·Microscopy Research and Technique·Giuseppe FamiliariSilvia Correr
Mar 28, 2006·Current Topics in Developmental Biology·Julian L Wong, Gary M Wessel
Sep 8, 2017·Journal of Cellular and Molecular Medicine·Su ZhouShixuan Wang
Jan 20, 2021·Journal of Clinical Pathology·Xiaoli WeiZhongxian Lu
Apr 4, 2021·International Journal of Molecular Sciences·Carla Moros-NicolásMª José Izquierdo-Rico
Jul 3, 2021·Reproductive Biomedicine Online·Su ZhouShixuan Wang

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