Subcellular dynamics of T cell immunological synapses and kinapses in lymph nodes.

Proceedings of the National Academy of Sciences of the United States of America
Georges A AzarPhilippe Bousso

Abstract

In vitro studies have revealed that T cell activation occurs during the formation of either dynamic or stable interactions with antigen-presenting cells (APC), and the respective cell junctions have been referred to as immunological kinapses and synapses. However, the relevance and molecular dynamics of kinapses and synapses remain to be established in vivo. Using two-photon imaging, we tracked the distribution of LAT-EGFP molecules during antigen recognition by activated CD4(+) T cells in lymph nodes. At steady state, LAT-EGFP molecules were preferentially found at the uropod of rapidly migrating T cells. In contrast to naïve T cells that fully stopped upon systemic antigen delivery, recently activated T cells decelerated and formed kinapses, characterized by continuous extension of membrane protrusions and by the absence of persistent LAT-EGFP clustering. On the other hand, activated CD4(+) T cells formed stable immunological synapses with antigen-loaded B cells and displayed sustained accumulation of LAT-EGFP fluorescence at the contact zone. Our results show that the state of T cell activation and the type of APC largely influence T cell-APC contact dynamics in lymph nodes. Furthermore, we provide a dynamic look at immunolo...Continue Reading

References

Jul 7, 1997·The Journal of Experimental Medicine·M NietoF Sánchez-Madrid
Mar 20, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·P ReichertM K Jenkins
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·C Gómez-MóutonC Martínez-A
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Q WangN Killeen
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Elena V TibaldiEllis L Reinherz
Jun 8, 2002·Science·Sabine StollRonald N Germain
Sep 28, 2002·Nature Immunology·Dorian B McGavernMichael B A Oldstone
Oct 3, 2002·The Journal of Cell Biology·Stephen C BunnellLawrence E Samelson
Jun 6, 2003·Current Opinion in Immunology·Alain Trautmann, Salvatore Valitutti
Nov 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mustapha FaroudiSalvatore Valitutti
Jan 9, 2004·Nature·Thorsten R MempelUlrich H Von Andrian
Oct 7, 2004·The Journal of Experimental Medicine·Mark J MillerMichael D Cahalan
Nov 2, 2004·Nature Immunology·Stéphanie HuguesSebastian Amigorena
Mar 9, 2005·The Journal of Biological Chemistry·Hidehiko ShogomoriDeborah A Brown
May 24, 2005·European Journal of Immunology·Cédric BrossardAlain Trautmann
Jul 13, 2005·The Journal of Cell Biology·Joseph LinAndrey S Shaw
Oct 6, 2005·The Journal of Cell Biology·Katharina GausThomas Harder
Oct 12, 2005·The Journal of Experimental Medicine·Gabriele CampiMichael L Dustin
Nov 3, 2005·Seminars in Immunology·Michael D Cahalan, Ian Parker
Aug 15, 2006·Nature Structural & Molecular Biology·Jon C D HoutmanLawrence E Samelson
Aug 23, 2006·The Journal of Experimental Medicine·Carlos BarciaPedro R Lowenstein
Jan 30, 2007·Nature Reviews. Immunology·Daniel D BilladeauTimothy S Gomez
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Zacarias GarciaPhilippe Bousso
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sindy H WeiIan Parker
Oct 6, 2007·Science·Kamal M KhannaLeo Lefrançois
Jan 5, 2008·Annual Review of Immunology·Michael D Cahalan, Ian Parker
Feb 16, 2008·Immunological Reviews·Michael L Dustin
Feb 16, 2008·Immunological Reviews·Susanna CelliPhilippe Bousso
Jul 5, 2008·Annual Review of Cell and Developmental Biology·Michael L Dustin
Aug 20, 2008·Nature Immunology·Peter Friedl, Bettina Weigelin

❮ Previous
Next ❯

Citations

Apr 15, 2010·Journal of Clinical Immunology·Matthew F Krummel, Michael D Cahalan
Oct 20, 2010·Immunology and Cell Biology·Heather J MelicharEllen A Robey
Jun 22, 2010·Nature Immunology·Matthew F Krummel
Dec 24, 2010·Nature Reviews. Immunology·Lakshmi BalagopalanLawrence E Samelson
Jun 30, 2012·Science·Ronald N GermainMichael D Cahalan
Apr 9, 2010·Journal of Virology·Walther MothesPeng Zhong
Feb 19, 2013·Proceedings of the National Academy of Sciences of the United States of America·Wilmara Salgado-PabónArmelle Phalipon
Feb 28, 2014·Seminars in Immunopathology·Tim Lämmermann, Ronald N Germain
Sep 6, 2014·Nature Reviews. Immunology·Steven L Reiner, William C Adams
Sep 11, 2010·FEBS Letters·Rémi Lasserre, Andrés Alcover
Jan 8, 2016·Biomolecular Concepts·Danielle R J VerboogenGeert van den Bogaart
Oct 14, 2010·FEBS Letters·Salvatore ValituttiLoïc Dupré
Apr 2, 2013·Progress in Retinal and Eye Research·John V ForresterMatthew E Wikstrom
Dec 4, 2012·Trends in Immunology·Yvonne J YamanakaJ Christopher Love
Mar 1, 2012·Gastroenterology·Manon E WildenbergDaniel W Hommes
Dec 5, 2015·Frontiers in Immunology·Loïc DupréJavier Rey-Barroso
Aug 2, 2011·European Journal of Immunology·Guido H WabnitzYvonne Samstag
Oct 15, 2013·Immunological Reviews·Kole T RoybalChristoph Wülfing
Aug 21, 2013·Immunological Reviews·E Ashley Moseman, Dorian B McGavern
Aug 21, 2013·Immunological Reviews·Thomas J Braciale, Young S Hahn
Sep 8, 2010·European Journal of Immunology·Julian StorimPeter Friedl
Sep 16, 2015·Proceedings of the National Academy of Sciences of the United States of America·Hélène D MoreauPhilippe Bousso
Sep 18, 2015·Frontiers in Immunology·Marinos KallikourdisFederica Benvenuti
Feb 22, 2014·Current Opinion in Immunology·Hélène D Moreau, Philippe Bousso
Mar 7, 2015·Immunology and Cell Biology·Joanna HalkiasHeather J Melichar
May 18, 2013·Biochimica Et Biophysica Acta·Sudha KumariMichael L Dustin
Oct 1, 2014·Immunology and Cell Biology·Izaskun MitxitorenaCarlos Barcia
Jul 17, 2014·Nature Reviews. Microbiology·Oliver T FacklerThorsten R Mempel
Nov 24, 2012·Nature Reviews. Immunology·Philippe Bousso, Hélène D Moreau
Jun 17, 2016·Molecules and Cells·Tara Capece, Minsoo Kim
Feb 12, 2020·Journal of Cell Science·Jorge Luis Galeano NiñoMaté Biro
Apr 24, 2018·Frontiers in Immunology·Víctor Calvo, Manuel Izquierdo
Dec 7, 2018·Frontiers in Immunology·Jérémie RossyDaniel F Legler
Aug 9, 2019·Proceedings of the National Academy of Sciences of the United States of America·Rong MaKhalid Salaita
Dec 19, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Heather J MelicharEllen A Robey
Nov 15, 2018·Frontiers in Cell and Developmental Biology·Noa Beatriz Martín-CófrecesFrancisco Sánchez-Madrid

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.