PMID: 3748272Jul 1, 1986Paper

Subcellular fractionation and distribution of cholinergic binding sites in fetal human brain

Neurochemical Research
J WhyteS Wonnacott

Abstract

Conventional subcellular fractionation techniques have been applied to human fetal brain (13-15 weeks gestation) and the fractions have been characterized by assaying for marker enzymes, cholinergic binding sites and electron microscopy. Fractionation of the homogenate resulted in a nuclear pellet (P1), a crude mitochondrial pellet (P2) and a supernatant (S2). Further resolution of the P2 fraction by density gradient centrifugation resulted in two bands at the gradient interfaces and a pellet. The P2 and subsequently the P2B fraction contained intact plasma membrane profiles as judged by the predominance of adenylate cyclase activity and the presence of occluded lactate dehydrogenase which constituted over 70% of the total activity in these fractions. Morphological examination of the gradient fractions revealed that the P2B fraction contains membrane bound structures which resemble synaptosomes prepared from neonatal rat brain. These structures have a granular matrix in which mitochondria and frequently, neurofilaments were observed. Very few synaptic vesicles were present and there was no evidence for post synaptic attachments. The cholinergic markers choline acetyltransferase, acetylcholinesterase and receptor sites defined b...Continue Reading

References

Dec 1, 1979·Journal of Microscopy·J B Forsdyke
Apr 1, 1977·Journal of Neurosurgery·M A Furman, K Shulman
Jan 1, 1978·Progress in Neurobiology·E Heilbronn, T Bartfai
Dec 20, 1974·Brain Research·R E GareyR G Heath
Feb 1, 1973·Journal of Neurochemistry·P D SwansonW L Stahl
Feb 28, 1973·Brain Research·M E MolliverH van der Loos
Oct 1, 1973·Archives of Disease in Childhood·J Dobbing, J Sands
Oct 28, 1970·Brain Research·F J Barrantes, G G Lunt
Feb 25, 1972·Brain Research·H SavolainenL E Brody
Jan 1, 1970·Zeitschrift für Zellforschung und mikroskopische Anatomie·D G Jones, E Revell
Jul 1, 1967·The Biochemical Journal·R M Marchbanks
Mar 1, 1983·Journal of Neurochemistry·J A HardyA M Kidd
Dec 13, 1982·Neuroscience Letters·P R HuttenlocherH Van der Loos
Jan 1, 1961·The Journal of Biophysical and Biochemical Cytology·E DE ROBERTISC J GOMEZ
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE
Jan 1, 1985·Neurochemistry International·C RapierS Wonnacott
Jan 1, 1985·Neurochemistry International·J S Aguilar, G G Lunt
Jan 1, 1985·Neurochemistry International·J WhyteS Wonnacott

❮ Previous
Next ❯

Citations

Dec 13, 1988·Brain Research·N J Cairns, S Wonnacott

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.