PMID: 7433896Jan 1, 1980Paper

Subcellular fractionation of human intestinal mucosa by large-scale zonal centrifugation. I. Characterization of subcellular organelles in the distal part of the ileum

Scandinavian Journal of Gastroenterology
C TagessonR Sjödahl

Abstract

The subcellular organelles in human ileal mucosa have been characterized by large-scale rate zonal and isopycnic density-gradient centrifugation. Mucosal scrapings were obtained from surgical specimens and the cells disrupted by extrusion under controlled pressure. The homogenized cells were then centrifuged to prepare a cell extract and the extract fractionated in a single-step procedure by zonal centrifugation on a continuous sucrose gradient. The subcellular organelles (brush borders, basal-lateral membranes, lysosomes, mitochondria, and endoplasmic reticulum) were located in the density gradient by assay of marker enzymes. Since a large number of analyses can be done on each fraction of interest, this fractionation technique may be used to study a variety of compounds and concepts involved in the subcellular physiology and pathology of human ileal mucosa.

References

Jan 1, 1976·Biochemical Society Transactions·J Tilleray, T J Peters
Sep 27, 1973·Biochimica Et Biophysica Acta·J SchmitzR K Crane
Aug 1, 1974·Molecular and Cellular Biochemistry·R Wattiaux
May 24, 1974·Biochimica Et Biophysica Acta·J SchmitzB Hadorn
Sep 6, 1974·Biochimica Et Biophysica Acta·H PreiserJ J Cerda
Sep 27, 1973·Biochimica Et Biophysica Acta·D MaestracciR K Crane

Citations

Nov 1, 1984·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C Tagesson, C Edling
May 1, 1988·Scandinavian Journal of Gastroenterology·C GustafsonC Tagesson
Jun 1, 1988·Scandinavian Journal of Clinical and Laboratory Investigation·M LindahlC Tagesson
Jan 1, 1985·Scandinavian Journal of Gastroenterology·C Tagesson, R Sjödahl

Related Concepts

Cell Fractionation
Centrifugation, Isopycnic
Centrifugation, Zonal
Endoplasmic Reticulum
Cytochemistry
Ileum
Structure of Intestinal Gland
Lysosomes
Microvilli
Mitochondria

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