Subcellular localisation of the p40phox component of NADPH oxidase involves direct interactions between the Phox homology domain and F-actin.

The International Journal of Biochemistry & Cell Biology
Dongmin ShaoLodewijk V Dekker

Abstract

Cytosolic components of the NADPH oxidase interact with the actin cytoskeleton. These interactions are thought to be important for the activation of this enzyme system but they are poorly characterised at the molecular level. Here we have explored the interaction between the actin cytoskeleton and p40(phox), one of the cytosolic components of NADPH oxidase. Full length p40(phox) expressed in COS cells co-localised with F-actin in a peripheral lamellar compartment. The co-localisation was lost after deletion of the Phox homology (PX) domain and the PX domain in isolation (p40PX) showed the same F-actin co-localisation as the full length protein. PX domains are known lipid-binding modules however, a mutant p40PX which did not bind lipids still co-localised with F-actin suggesting that lipid-independent interactions underlie the localisation. Affinity chromatography identified actin as a binding partner for p40PX in neutrophil extracts. Pure actin interacted with both p40(phox) and with p40PX suggesting it is a direct interaction. Disruption of the actin cytoskeleton with cytochalasin D resulted in actin rearrangement and concomitantly the localisation of full length p40(phox) proteins and that of p40PX changed. Thus p40PX is a du...Continue Reading

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Citations

Dec 1, 2012·Current Opinion in Cell Biology·Jonathan R Terman, Anna Kashina
Feb 7, 2012·Seminars in Cell & Developmental Biology·Eric K OermannYue Xiong
Oct 26, 2013·Journal of Receptor and Signal Transduction Research·Song Mao, Songming Huang
May 31, 2011·Free Radical Biology & Medicine·Tim Van AsschePaul Cos
Nov 19, 2017·The Journal of Cell Biology·Darko StojkovShida Yousefi
Jul 1, 2020·Journal of Pineal Research·Somanathapura K NaveenKumarKesturu S Girish
Mar 31, 2020·Photosynthesis Research·Arkajo Majumdar, Rup Kumar Kar
May 7, 2020·Free Radical Biology & Medicine·Aslihan Terzi, Daniel M Suter

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Methods Mentioned

BETA
GTPase
gel filtration
Transfection
flow chromatography
immunoprecipitation
two hybrid

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