PMID: 9169609May 1, 1997Paper

Subcellular localization and function of melanogenic enzymes in the ink gland of Sepia officinalis

The Biochemical Journal
A PalumboV J Hearing

Abstract

The ink gland of the cuttlefish Sepia officinalis has traditionally been regarded as a convenient model system for investigating melanogenesis. This gland has been shown to contain a variety of melanogenic enzymes including tyrosinase, a dopachrome-rearranging enzyme and peroxidase. However, whether and to what extent these enzymes co-localize in the melanogenic compartments and interact is an open question. Using polyclonal antibodies that recognize the corresponding Sepia proteins, we have been able to demonstrate that peroxidase has a different subcellular localization pattern from tyrosinase and dopachrome-rearranging enzyme. Whereas peroxidase is located in the rough endoplasmic reticulum and in the matrix of premelanosomes and melanosomes, tyrosinase and dopachrome-rearranging enzyme are present in the rough endoplasmic reticulum-Golgi transport system, at the level of trans-Golgi cisternae, trans-Golgi network and coated vesicles, and in melanosomes on pigmented granules. These results fill a longstanding gap in our knowledge of the melanin-producing system in Sepia and provide the necessary background for dissection at the molecular level of the complex interaction between melanogenic enzymes. Moreover, the peculiar and...Continue Reading

Citations

Sep 27, 2003·European Journal of Biochemistry·Tetsushi NaraokaAtsuo Kimura
Aug 7, 2003·Biochemical and Biophysical Research Communications·Gian Luigi RussoAnna Palumbo
Jun 28, 2005·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Anna Palumbo
Jan 25, 2006·Melanoma Research·Rangaprasad Sarangarajan, Shireesh P Apte
Mar 15, 2002·Nature·Norimichi Kitagawa, Shigeru Ichihara
Feb 16, 1999·Nature·M S GrazianoC G Gross
Jan 18, 2007·Journal of the Association for Research in Otolaryngology : JARO·Zachary M Smith, Bertrand Delgutte
Sep 3, 2003·Pigment Cell Research·Anna Palumbo
Nov 20, 2020·Genetics and Molecular Biology·Andrés Posso-Terranova, José Andrés
Dec 16, 2020·Marine Drugs·Vida ŠimatFatih Özogul
Feb 19, 2008·The Journal of Experimental Biology·Teresita C Insausti, Jérôme Casas

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