Subcellular localization and functional analyses of a PR10 protein gene from Vitis pseudoreticulata in response to Plasmopara viticola infection.

Protoplasma
Mingyang HeZhenfang Fu

Abstract

Downy mildew, caused by the oomycete Plasmopara viticola, is a serious fungal disease in the cultivated European grapevines (Vitis vinifera L.). The class 10 of pathogenesis-related (PR) genes in grapevine leaves was reported to be accumulated at mRNA level in response to P. viticola infection. To elucidate the functional roles of PR10 genes during plant-pathogen interactions, a PR10 gene from a fungal-resistant accession of Chinese wild Vitis pseudoreticulata (designated VpPR10.2) was isolated and showed high homology to PR10.2 from susceptible V. vinifera (designated VvPR10.2). Comparative analysis displayed that there were significant differences in the patterns of gene expression between the PR10 genes from the two host species. VpPR10.2 was induced with high level in leaves infected by P. viticola, while VvPR10.2 showed a low response to this inoculation. Recombinant VpPR10.2 protein showed DNase activity against host genomic DNA and RNase activity against yeast total RNA in vitro. Meanwhile, recombinant VpPR10.2 protein inhibited the growth of tobacco fungus Alternaria alternata and over-expression of VpPR10.2 in susceptible V. vinifera enhanced the host resistance to P. viticola. The results from subcellular localization...Continue Reading

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Jul 6, 2012·Molecular Biotechnology·Parinita AgarwalJitendra Chikara
Nov 29, 2014·Plant Biotechnology Journal·Noémie S JellyPascale Maillot
May 6, 2016·Frontiers in Plant Science·Katja KarppinenAnja Hohtola
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Jan 8, 2021·Environmental Science and Pollution Research International·Vinay Kumar, Shiv Kumar Dwivedi
Mar 30, 2021·Plant Science : an International Journal of Experimental Plant Biology·Kentaro Fujita, Hideyuki Inui

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