Subcellular localization and membrane topology of the melon ethylene receptor CmERS1.

Plant Physiology
Biao MaHiroshi Ezura

Abstract

Ethylene receptors are multispanning membrane proteins that negatively regulate ethylene responses via the formation of a signaling complex with downstream elements. To better understand their biochemical functions, we investigated the membrane topology and subcellular localization of CmERS1, a melon (Cucumis melo) ethylene receptor that has three putative transmembrane domains at the N terminus. Analyses using membrane fractionation and green fluorescent protein imaging approaches indicate that CmERS1 is predominantly associated with the endoplasmic reticulum (ER) membrane. Detergent treatments of melon microsomes showed that the receptor protein is integrally bound to the ER membrane. A protease protection assay and N-glycosylation analysis were used to determine membrane topology. The results indicate that CmERS1 spans the membrane three times, with its N terminus facing the luminal space and the large C-terminal portion lying on the cytosolic side of the ER membrane. This orientation provides a platform for interaction with the cytosolic signaling elements. The three N-terminal transmembrane segments were found to function as topogenic sequences to determine the final topology. High conservation of these topogenic sequences...Continue Reading

References

Sep 7, 1976·Biochimica Et Biophysica Acta·D J Bowles, H Kauss
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E HartmannH F Lodish
Nov 4, 1985·Nature·M Friedlander, G Blobel
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G Blobel
Dec 1, 1994·Computer Applications in the Biosciences : CABIOS·M G Claros, G von Heijne
May 26, 1995·The Journal of Biological Chemistry·G E SchallerA B Bleecker
Mar 1, 1996·Current Biology : CB·W ChiuJ Sheen
Aug 1, 1996·Protein Science : a Publication of the Protein Society·B RostR Casadio
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·J HaseloffS Hodge
Jan 20, 1998·Trends in Biochemical Sciences·L Aravind, C P Ponting
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·K L ClarkC Chang
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·R L GambleG E Schaller
Oct 17, 1998·The Journal of Biological Chemistry·K OtaK Mihara
Nov 4, 1998·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A B BleeckerB M Binder
Feb 12, 1999·Science·F I RodríguezA B Bleecker
Nov 27, 1999·The Journal of Biological Chemistry·A DevotoP Schulze-Lefert
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·M van Geest, J S Lolkema
Jul 20, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C Chang, R Stadler
Feb 16, 2002·Journal of Experimental Botany·Hidenori TakahashiHiroshi Ezura
Mar 14, 2002·Biochemical and Biophysical Research Communications·Koutarou UkajiKatsuyoshi Mihara
Mar 28, 2002·The Journal of Biological Chemistry·Yi-Feng ChenG Eric Schaller
Apr 27, 2002·The Journal of Biological Chemistry·Marçal VilarIsmael Mingarro
Jul 1, 1996·The Plant Cell·N. Ferrol, A. B. Bennett
Jan 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Wuyi WangAnthony B Bleecker
Jan 22, 2003·The Plant Journal : for Cell and Molecular Biology·Yafan HuangJoseph J Kieber
Dec 9, 2003·Journal of Plant Physiology·Toshiyuki OgawaraHiroshi Ezura
Dec 24, 2003·The Plant Journal : for Cell and Molecular Biology·Andrew W McCartneyRobert T Mullen
Jan 21, 2004·Current Opinion in Plant Biology·Hongwei Guo, Joseph R Ecker
Feb 11, 2005·The Plant Journal : for Cell and Molecular Biology·Ronan C O'MalleyAnthony B Bleecker

❮ Previous
Next ❯

Citations

Mar 26, 2013·Plant Cell Reports·Feifei WangChun-Hai Dong
Oct 19, 2010·The Journal of Biological Chemistry·Chun-Hai DongCaren Chang
Sep 30, 2010·The Journal of Biological Chemistry·Brad M BinderAnthony B Bleecker
Jul 2, 2009·Journal of Experimental Botany·Zhefeng LinDon Grierson
Jul 17, 2012·Plant Physiology·Yusuke KamiyoshiharaHarry J Klee
Oct 20, 2012·Genetics and Molecular Research : GMR·C A Contreras-VergaraM A Islas-Osuna
Mar 20, 2016·Plant Science : an International Journal of Experimental Plant Biology·Patrick WinterhagenJens N Wünsche
Mar 7, 2008·Critical Reviews in Biotechnology·Hector G Nuñez-PaleniusDaniel J Cantliffe
Aug 12, 2008·Current Opinion in Plant Biology·Mandy D Kendrick, Caren Chang
Nov 15, 2007·The Plant Journal : for Cell and Molecular Biology·Chun-Hai DongCaren Chang
Mar 19, 2014·The Plant Journal : for Cell and Molecular Biology·Jianhong ChangCaren Chang
May 31, 2015·Plant Science : an International Journal of Experimental Plant Biology·Hernán RojasAriel Goldraij
Oct 8, 2013·Plant Physiology and Biochemistry : PPB·Noushina IqbalNafees A Khan
Feb 22, 2018·Proceedings of the National Academy of Sciences of the United States of America·Biao MaJin-Song Zhang
Jan 25, 2018·Journal of Experimental Botany·Yanling WangTianlai Li
Jun 22, 2019·Scientific Reports·Stephan Schott-VerdugoGeorg Groth
Apr 26, 2020·The Journal of Biological Chemistry·Brad M Binder
Oct 24, 2020·Journal of Integrative Plant Biology·He ZhaoJin-Song Zhang
Apr 20, 2020·Analytical Biochemistry·Sergey N Lomin, Georgy A Romanov
Mar 30, 2021·Frontiers in Microbiology·Tianlu HangTing Ding

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.