PMID: 7545519May 1, 1995Paper

Subcellular localization and molecular pharmacology of distinct populations of [3H]-AMPA binding sites in rat hippocampus

British Journal of Pharmacology
J M Henley

Abstract

1. The subcellular distributions of [3H]-alpha-amino-3-hydroxy-5- methylisoxazolepropionate ([3H]-AMPA) and [3H]-kainate binding sites in rat hippocampus were investigated by cell fractionation techniques. 2. Two major populations of [3H]-AMPA sites were detected with the majority of binding located intracellularly in the microsomal (P3) fraction. Most of the remaining sites were in the synaptosomal membrane fraction but some were also present in the nuclear fraction. In contrast, essentially all of the [3H]-kainate binding sites were in the synaptosomal membrane fraction. 3. Saturation binding analysis yielded KD and Bmax values for [3H]-AMPA of 147 nM and 2.6 pmol mg-1 protein respectively for the synaptosomal membrane-associated sites and 129 nM and 5.3 pmol mg-1 protein respectively for the microsomal sites. 4. Both main populations of [3H]-AMPA sites displayed the same rank order of inhibition by competitive ligands, the apparent Mr values of GluR1 subunits were equivalent, suggesting the same degree of post-translational modification and the hydrodynamic properties of the receptor complexes were identical. 5. These data are consistent with the hypothesis that the movement of AMPA receptors between cellular compartments in...Continue Reading

References

Aug 1, 1992·Progress in Neurobiology·J M Barnes, J M Henley
Nov 1, 1992·Journal of Neurobiology·D W Choi
Apr 15, 1992·The Journal of Comparative Neurology·R S Petralia, R J Wenthold
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J M HenleyE A Barnard
Jul 1, 1992·Trends in Pharmacological Sciences·B Sommer, P H Seeburg
Feb 28, 1985·Neuroscience Letters·T Honoré, M Nielsen
Aug 1, 1993·Trends in Pharmacological Sciences·P H Seeburg
Jan 1, 1994·Annual Review of Neuroscience·M Hollmann, S Heinemann
Jan 7, 1993·Nature·T V Bliss, G L Collingridge

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Citations

Jan 15, 1999·Molecular Neurobiology·K K Dev, J M Henley
Feb 15, 1996·European Journal of Pharmacology·D PruneauP Bélichard
Mar 23, 2000·Neuropharmacology·S P BraithwaiteJ M Henley
Jan 29, 2000·Brain Research. Developmental Brain Research·E AkessonE Sundström
Jul 12, 2005·Neurochemistry International·P S PinheiroJ O Malva

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