Subcellular localization and protein interaction of the human LIMK2 gene expressing alternative transcripts with tissue-specific regulation

Biochemical and Biophysical Research Communications
H OsadaT Takahashi

Abstract

In our efforts to explore possible roles of proteins with a LIM domain, which is a cysteine-rich Zinc-binding motif, in differentiation and oncogenesis in the lung, we have cloned a human LIMK2 gene and identified two alternative transcripts, LIMK2a and LIMK2b, which are probably due to variation in transcriptional initiation. The former encodes a protein containing two LIM domains, a PDZ domain, and a kinase domain, while the latter has only one and half LIM domains. The predominance of the two transcripts appears to be regulated in a tissue-specific manner. Alteration of the regulation is also observed in some cancer cell lines. Transfection studies have shown an association of 63-kDa and 58-kDa proteins with the LIMK2a and LIMK2b protein; the former is distributed in the cytoplasm and nucleus and the latter occurs mainly in the cytoplasm and is scarcely translocated to the nucleus. In contrast, a truncated LIMK2-Kinase has a nuclear location, not showing the protein association.

Citations

Oct 7, 2004·Journal of Vascular Research·Shaner BongalonIlia A Yamboliev
Jan 11, 2001·Developmental Biology·T TakahashiT Nakamura
Jan 11, 2002·Developmental Biology·Hisaaki TakahashiToshikazu Nakamura
Nov 25, 2003·Biology of Reproduction·J A GuttmanA W Vogl
May 4, 1999·The International Journal of Biochemistry & Cell Biology·C A Stanyon, O Bernard
Nov 22, 1997·Molecular Endocrinology·M R SchillerB A Eipper
Oct 18, 2000·The Journal of Biological Chemistry·Y ZhangD M Center
Aug 26, 1998·Biochemical and Biophysical Research Communications·H TakahashiT Nakamura
Jan 27, 2015·Journal of Medicinal Chemistry·Yan YinYangbo Feng

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