Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases.

The Journal of Biological Chemistry
S HatakeyamaA M Weissman

Abstract

In most instances, the transfer of ubiquitin to target proteins is catalyzed by the action of ubiquitin protein ligases (E3s). Full-length cDNAs encoding murine E6-associated protein (mE6-AP) as well as Nedd-4, a protein that is homologous to E6-AP in its C terminus, were cloned. Nedd-4 and mouse E6-AP are both enzymatically active E3s and function with members of the UbcH5 family of E2s. Mouse E6-AP, like its human counterpart, ubiquitinates p53 in the presence of human papilloma virus E6 protein, while Nedd-4 does not. Consistent with its role in p53 ubiquitination, mE6-AP was found both in the nucleus and cytosol, while Nedd-4 was found only in the cytosol. Binding studies implicate a 150-amino acid region that is 40% identical between mE6-AP and Nedd-4 as a binding site for the C-terminal portion of an E2 enzyme (UbcH5B). Nedd-4 was determined to have a second nonoverlapping E2 binding site that recognizes the first 67 amino acids of UbcH5B but not the more C-terminal portion of this E2. These findings provide the first demonstration of physical interactions between mammalian E2s and E3s and establish that these interactions occur independently of ubiquitin and an intact E3 catalytic domain. Furthermore, the presence of two...Continue Reading

References

May 29, 1992·Cell·A Varshavsky
Jan 1, 1992·Annual Review of Biochemistry·A Hershko, A Ciechanover
Jan 1, 1992·Annual Review of Genetics·S Jentsch
Jun 30, 1992·Biochemical and Biophysical Research Communications·S KumarM Noda
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R J DohmenA Varshavsky
Dec 1, 1985·Molecular and Cellular Biology·G I EvanJ M Bishop
Apr 1, 1995·Current Opinion in Cell Biology·M Hochstrasser
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·J M HuibregtseP M Howley
Jan 23, 1995·FEBS Letters·K Hofmann, P Bucher
Feb 24, 1995·Cell·D Thanos, T Maniatis
Oct 7, 1994·Cell·A Ciechanover
Jun 15, 1994·The Biochemical Journal·S J GrenfellA L Schwartz
Sep 2, 1994·Science·K Madura, A Varshavsky
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·M ScheffnerP M Howley
Jan 1, 1995·Annual Review of Nutrition·K D Wilkinson
Dec 22, 1995·The Journal of Biological Chemistry·J P JensenA M Weissman
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·O V KovalenkoE I Golub
Apr 12, 1996·The Journal of Biological Chemistry·C CenciarelliA M Weissman
Jan 1, 1996·Annual Review of Biochemistry·O CouxA L Goldberg
Jan 1, 1996·Annual Review of Genetics·M Hochstrasser
Apr 1, 1997·Immunology Today·A M Weissman

❮ Previous
Next ❯

Citations

Sep 22, 2000·Trends in Cell Biology·P K JacksonJ D Reimann
Dec 14, 2011·Journal of Proteome Research·Gozde KarAttila Gursoy
Oct 11, 2007·Nature Reviews. Neuroscience·Lawrence S WilkinsonAnthony R Isles
Feb 12, 2008·Nature Structural & Molecular Biology·Danny T HuangBrenda A Schulman
Oct 22, 2002·Molecular Biology of the Cell·Ikuo NakamichiKeiichi I Nakayama
Oct 18, 2000·The EMBO Journal·S M Plafker, I G Macara
Feb 6, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fenghua ZengRaymond C Harris
Mar 3, 2004·Molecular and Cellular Biology·Christophe Debonneville, Olivier Staub
Oct 6, 1998·Annual Review of Biochemistry·A Hershko, A Ciechanover
Jun 8, 2001·Annual Review of Biochemistry·C M Pickart
Jan 19, 1999·Annual Review of Cell and Developmental Biology·J S Bonifacino, A M Weissman
Apr 16, 2009·Journal of Cell Science·Zlatka KostovaAllan M Weissman
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·S HatakeyamaK Nakayama
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·K L LorickA M Weissman
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bo ChenAllan M Weissman
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y ZhangR Derynck
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·W K VersawE H Bresnick
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z NawazB W O'Malley
Apr 25, 2000·Journal of Molecular and Cellular Cardiology·S E JohnattyM Abdellatif
Mar 25, 2009·The Plant Journal : for Cell and Molecular Biology·Sabine RaabStefan Hoth
Nov 2, 2006·Molecular Microbiology·Ying ZhangDaoguo Zhou
Dec 13, 2005·The International Journal of Biochemistry & Cell Biology·Andrew B FotiaSharad Kumar
Aug 2, 2003·Biochemical and Biophysical Research Communications·Kou MiyazakiAkira Nakagawara
Jul 21, 2015·Cell Reports·Jessica M PerezDerek W Abbott
Nov 18, 2008·Virology·Paola Blanchette, Philip E Branton
Jul 2, 1998·Molecular and Cellular Neurosciences·J D WoodC A Ross
Jan 7, 2005·Journal of Biomolecular Screening·I V DavydovK H Vousden
Aug 9, 2003·The Journal of Biological Chemistry·Alessandra MagnificoAllan M Weissman
Mar 27, 2007·The Biochemical Journal·Dominik WiemuthFiona J McDonald
Nov 12, 2015·The Biochemical Journal·Robert D'CruzJane A E Batt
Nov 22, 2001·The Journal of Biological Chemistry·Rodolfo MurillasMichael R Kuehn

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.