Aug 1, 1976

Subcellular localization of a rat liver enzyme converting thyroxine into tri-iodothyronine and possible involvement of essential thiol groups

The Biochemical Journal
T J VisserGeorg Hennemann

Abstract

Experiments with rat liver homogenates showed that on subcellular fractionation the ability to catalyse the conversion of thyroxine into tri-iodothyronine was lost. The activity could in part be restored by addition of the cytosol to the microsomal fraction. Both components were found to be heat labile. The necessity of the presence of cytosol could be circumvented by incorporation of thiol-group-containing compounds in the medium. Optimal enzymic activity was observed in the presence of dithiothreitol and EDTA in medium of low osmolarity. By comparing the distribution of the converting enzyme over the subcellular fractions with a microsomal marker enzyme, glucose 6-phosphatase, it was demonstrated that the former is indeed of microsomal origin. Finally, it was shown that thiol groups play an essential role in the conversion of thyroxine into tri-iodothyronine.

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Mentioned in this Paper

Establishment and Maintenance of Localization
Mercury
4-Chloromercuribenzenesulfonate
Novothyral
Glucosephosphatase
Osmolarity Measurement
Isocyanides
Thyroxine Measurement
Sputolysin
Dithiothreitol

About this Paper

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