Subcellular localization of alpha-subunits of trimeric G-proteins in human platelets

Biochemical and Biophysical Research Communications
A N GiesbertsG van Willigen

Abstract

Following subcellular fractionation of platelet homogenates and Western blotting, two groups of alpha-subunits of trimeric G-proteins could be distinguished. Group 1 consisted of alpha(i)-2, alpha(i)-3, alpha(z), alpha(s), and alpha(q) and was predominantly localized in membranes. Group 2 consisted of alpha16 and alph12 and was predominantly localized in the cytosol. Plasma membranes and dense tubular system (DTS)-membranes showed the same distribution of Group 1 alpha-subunits. An exception was alpha(q), which was virtually absent in the DTS as were Group 2 subunits. In addition, this compartment showed a doublet for alpha(z). Group 1 alpha-subunits were also found in fractions with the combined secretory granules and in separate dense granules. In addition, alpha16 was found in these granule fractions, but secretion granules were devoid of alpha12. These data reveal a heterogeneous distribution of alpha-subunits in platelet compartments and may indicate that G12 and G16 play different roles in platelets than members of the G(i) and G(s) classes and other members of the G(q) class.

Citations

Apr 8, 2010·Journal of Biomedical Science·Harold J Ting, Fadi T Khasawneh
Aug 11, 2000·Biological Chemistry·S Offermanns
Dec 6, 2000·European Journal of Biochemistry·G van WilligenJ W Akkerman
Aug 26, 1998·Biochimica Et Biophysica Acta·J L Stow, K Heimann
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·E L Watson
Sep 23, 1997·Biochemical and Biophysical Research Communications·E L WatsonD H Dijulio

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