PMID: 8985428Jan 1, 1997Paper

Subcellular localization of avian sarcoma virus and human immunodeficiency virus type 1 integrases

Journal of Virology
G KukoljA M Skalka

Abstract

The composition and subcellular trafficking of subviral preintegration complexes are reported to vary among the different retroviruses. The process by which the avian sarcoma virus (ASV) preintegration complex gains access to target chromatin remains unknown. Here we report that ASV integrase (IN) expressed as a fusion to beta-galactosidase accumulates in the nuclei of transfected COS-1 cells. In contrast, human immunodeficiency type 1 (HIV-1) IN-beta-galactosidase fusions expressed similarly are predominantly cytoplasmic. To identify the region of ASV IN that specifies nuclear localization, various subdomains of the protein were expressed as beta-galactosidase fusions and their subcellular locations were assessed cytochemically and by indirect immunofluorescence. These analyses showed that the ASV IN protein possesses a functional nuclear localization signal that spans amino acids 206 to 235 and displays limited homology with known nuclear transport signals.

References

Jan 1, 1992·Annual Review of Genetics·S P Goff
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M I BukrinskyM Stevenson
Dec 1, 1991·Trends in Biochemical Sciences·C Dingwall, R A Laskey
Feb 25, 1991·Nucleic Acids Research·E KhanA M Skalka
Feb 8, 1991·Cell·P A Silver
Dec 1, 1990·Journal of Virology·Y M Lee, J M Coffin
Apr 1, 1989·Genes & Development·B BowermanH E Varmus
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B K FelberG N Pavlakis
Sep 1, 1974·Journal of Virology·E H Humphries, H M Temin
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E H HumphriesM E Reichmann
Jan 1, 1994·Annual Review of Biochemistry·R A Katz, A M Skalka
Sep 1, 1994·AIDS Research and Human Retroviruses·D Bizub-BenderA M Skalka
Sep 1, 1995·Nature Structural Biology·A P EijkelenboomK Hård
Oct 20, 1995·Journal of Molecular Biology·G BujaczA M Skalka
Aug 8, 1995·Biochemistry·P J LodiA M Gronenborn
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·U von SchwedlerD Trono
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·N K HeinzingerM Emerman
Aug 16, 1996·The Journal of Biological Chemistry·M D Andrake, A M Skalka
Jan 1, 1996·Trends in Cell Biology·M Stevenson

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Citations

Nov 22, 2000·The Journal of General Virology·H ImrichA Rethwilm
Sep 5, 2001·Journal of Virology·T Hatziioannou, S P Goff
May 22, 2002·Journal of Virology·Susanne WernerBirgitta M Wöhrl
Feb 17, 2007·Nature Reviews. Microbiology·Youichi Suzuki, Robert Craigie
Apr 14, 2004·Journal of Virology·James G GregerAnna Marie Skalka
Jan 18, 2006·Journal of Virology·Loraine Jarrosson-WuillemeAndrea Cimarelli
Feb 3, 1998·Molecular and Cellular Biology·S P MooreD J Garfinkel
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·P GallayD Trono
May 7, 2002·Journal of Virology·Richard A KatzAnna Marie Skalka
Oct 28, 2006·Retrovirology·Ariberto Fassati
Jul 23, 2013·Viruses·Md Alamgir HossainCha-Gyun Shin
Dec 16, 1998·Journal of Virology·M H Nymark-McMahon, S B Sandmeyer
Sep 18, 2003·Journal of Cell Science·Eric DevroePamela A Silver
Feb 26, 1998·Antiviral Research·E Asante-Appiah, A M Skalka
Aug 9, 2008·Retrovirology·Mark D AndrakeAnna Marie Skalka

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