Multispecific antigen-binding fragments (Fab) from rabbit antisera against rat very low density lipoproteins (VLDL) and Fab against rat low density lipoproteins that were monospecific for the B apoprotein were conjugated to horseradish peroxidase. Conjugates were incubated with 6-mum frozen sections from fresh and perfusion-fixed livers and with tissue chopper sections (40 mum thick) from perfusion-fixed livers. In the light microscope, specific reaction product was present in all hepatocytes of experimental sections as intense brown to black spots whose locations corresponded to the distribution of the Golgi apparatus: along the bile canaliculi, near the nuclei, and between the nuclei and bile canaliculi. Perfusion fixation with formaldehyde produced satisfactory ultrastructural preservation with retention of lipoprotein antigenic determinants. In the electron microscope, patches of cisternae and ribosomes of the rough endoplasmic reticulum (ER) and particularly its smooth-surfaced ends, vesicles located between the rough ER and the Golgi apparatus, the Golgi apparatus and its secretory vesicles and VLDL particles in the space of Disse all bore reaction product. The tubules and vesicles of typical hepatocyte smooth ER did not ...Continue Reading
Characterization of remnants produced during the metabolism of triglyceride-rich lipoproteins of blood plasma and intestinal lymph in the rat
Ultrastructural localization of intracellular immune globulins in plasma cells and lymphoblasts by enzyme-labeled antibodies
The use of peroxidase-labelled antiglobulin for ultrastructural localization of tissue antigens reacting with serum antibodies
Cofactor activity of protein components of human very low density lipoproteins in the hydrolysis of triglycerides by lipoproteins lipase from different sources
Assembly and secretion of very low density lipoproteins by rat liver following inhibition of protein synthesis with cycloheximide
A rapid electrophoretic technique for identification of subunit species of apoproteins in serum lipoproteins
Metabolism of rat plasma very low density lipoprotein. II. Fate in circulation of apoprotein subunits
Incorporation of sialic acid into sialidase-treated apolipoprotein of human, very low density lipoprotein by a pork liver sialytransferase
Light and electron microscopic localization of antigens in tissues embedded in polyethylene glycol with a peroxidase labeled antibody method
The secretory pathways of rat serum glycoproteins and albumin. Localization of newly formed proteins within the endoplasmic reticulum
Some studies on the metabolism of phospholipids in Golgi complex from bovine and rat liver in comparison to other subcellular fractions
Growth and differentiation of cytoplasmic membranes in the course of lipoprotein granule synthesis in the hepatic cell. I. Elaboration of elements of the Golgi complex
Lipid synthesis, intracellular transport, storage, and secretion. I. Electron microscopic radioautographic study of liver after injection of tritiated palmitate or glycerol in fasted and ethanol-treated rats
The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum
A cytochemical study on the pancreas of the guinea pig. 5. In vivo incorporation of leucine-1-C14 into the chymotrypsinogen of various cell fractions
Conformational control through translocational regulation: a new view of secretory and membrane protein folding
Secretion of lipid-poor nascent human apolipoprotein apoAI, apoCIII, and apoE by cell clones expressing the corresponding genes
A post-embedding immunoelectron-microscopic demonstration of apolipoprotein-B-containing lipoprotein particles in hepatocytes of fetal rats
Localization of apoVLDL-II, a major apoprotein in very low density lipoproteins, in the estrogen-treated cockerel liver by immunoelectron microscopy
Acute suppression of VLDL1 secretion rate by insulin is associated with hepatic fat content and insulin resistance
Transplantation of non-visceral fat to the visceral cavity improves glucose tolerance in mice: investigation of hepatic lipids and insulin sensitivity
Oleate acutely stimulates the secretion of triacylglycerol by cultured rat hepatocytes by accelerating the emptying of the secretory compartment
Regulation of HepG2 cell apolipoprotein B metabolism by the citrus flavanones hesperetin and naringenin
Ultrastructural immunogold labeling of lipid-laden enterocytes from patients with genetic malabsorption syndromes
Progress towards understanding the role of microsomal triglyceride transfer protein in apolipoprotein-B lipoprotein assembly
Distribution of microsomal triglyceride transfer protein within sub-endoplasmic reticulum regions in human hepatoma cells
Localization of neurophysin within organelles associated with protein synthesis and packaging in the hypothalamoneurohypophysial system: an immunocytochemical study
Immunocytochemical localization of albumin in the secretory apparatus of rat liver parenchymal cells
Secretion of apolipoprotein B-containing lipoproteins from HeLa cells is dependent on expression of the microsomal triglyceride transfer protein and is regulated by lipid availability
Murine mammary-derived cells secrete the N-terminal 41% of human apolipoprotein B on high density lipoprotein-sized lipoproteins containing a triacylglycerol-rich core
Peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) stimulates VLDL assembly through activation of cell death-inducing DFFA-like effector B (CideB).
Biosynthesis of high density lipoprotein by chicken liver: intracellular transport and proteolytic processing of nascent apolipoprotein A-1
The G protein of vesicular stomatitis virus has free access into and egress from the smooth endoplasmic reticulum of UT-1 cells
Lipogenesis and the synthesis and secretion of very low density lipoprotein by avian liver cells in nonproliferating monolayer culture. Hormonal effects
Relationship between the Golgi apparatus, GERL, and secretory granules in acinar cells of the rat exorbital lacrimal gland
Binding and uptake of 125I-insulin into rat liver hepatocytes and endothelium. An in vivo radioautographic study
Biosynthesis of high density lipoprotein by chicken liver: nature of nascent intracellular high density lipoprotein
Biosynthesis of high density lipoprotein by chicken liver: conjugation of nascent lipids with apoprotein A1
Palmitoylation of apolipoprotein B is required for proper intracellular sorting and transport of cholesteroyl esters and triglycerides
Understanding coronary heart disease as a consequence of defective regulation of apolipoprotein B metabolism
Biogenesis of very-low-density lipoproteins in rat liver. Intracellular distribution of apolipoprotein B
Epitopes of apolipoprotein B-100 and B-48 in both liver and intestine. Expression and evidence for local synthesis in recessive abetalipoproteinemia
Regulation of biliary cholesterol secretion. Functional relationship between the canalicular and sinusoidal cholesterol secretory pathways in the rat
A genetic model for absent chylomicron formation: mice producing apolipoprotein B in the liver, but not in the intestine
Disruption of LDL receptor gene in transgenic SREBP-1a mice unmasks hyperlipidemia resulting from production of lipid-rich VLDL
Analysis of the role of microsomal triglyceride transfer protein in the liver of tissue-specific knockout mice
Apolipoprotein B-containing lipoprotein assembly in microsomal triglyceride transfer protein-deficient McA-RH7777 cells.
Opposing roles of cell death-inducing DFF45-like effector B and perilipin 2 in controlling hepatic VLDL lipidation.
Structural and dynamic interfacial properties of the lipoprotein initiating domain of apolipoprotein B.
Effects of Therapeutic Lifestyle Change diets high and low in dietary fish-derived FAs on lipoprotein metabolism in middle-aged and elderly subjects.
Development and partial characterisation of an antiserum against apolipoprotein B of the short-finned eel, Anguilla australis
Qualitative and quantitative changes in hepatic lipoprotein particles following acute injury of the rat liver induced by thioacetamide
Identification of two regions in apolipoprotein B100 that are exposed on the cytosolic side of the endoplasmic reticulum membrane
The hepatic secretion of lecithin: cholesterol acyltransferase in rats with increased secretion of triglycerides due to ventromedial hypothalamic lesions
Proteolytic processing and compartmentalization of the primary translation products of mammalian apolipoprotein mRNAs
ORP10, a cholesterol binding protein associated with microtubules, regulates apolipoprotein B-100 secretion
Apolipoprotein B: a clinically important apolipoprotein which assembles atherogenic lipoproteins and promotes the development of atherosclerosis
Diacylglycerol acyltransferase 2 acts upstream of diacylglycerol acyltransferase 1 and utilizes nascent diglycerides and de novo synthesized fatty acids in HepG2 cells
Phospholipid transfer activity of microsomal triglyceride transfer protein produces apolipoprotein B and reduces hepatosteatosis while maintaining low plasma lipids in mice
Increased level of apolipoprotein B mRNA in the liver of ventromedial hypothalamus lesioned obese rats
Immunoelectronmicroscopical investigations on the adsorptive endocytosis of low density lipoproteins by human fibroblasts
Studies on the cellular toxicity of polychlorinated biphenyls (PCBs) partial block and alteration of intracellular migration of lipoprotein particles in rat liver
Studies on fatty liver with isolated hepatocytes. II. The action of carbon tetrachloride on lipid peroxidation, protein, and triglyceride synthesis and secretion
Continuity of intracellular channels with extracellular space in adipose tissue and liver: demonstrated with tannic acid and lanthanum
Separation of the different classes of intrahepatic lipoproteins from various animal species. Their binding with 2,3,7,8-tetrachlorodibenzo-p-dioxin and benzo(a)pyrene
Hepatic triacylglycerol synthesis and secretion: DGAT2 as the link between glycaemia and triglyceridaemia
Golgi fractions from livers of control and ethanol-intoxicated rats. Enzymic and morphologic properties following rapid isolation
Isolation of Golgi fractions from colchicine-treated rat liver. I. Morphological and enzymic characterization
Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis
Visualization of the uptake and processing of oxidized low-density lipoproteins in human and rat liver
A new combined multicompartmental model for apolipoprotein B-100 and triglyceride metabolism in VLDL subfractions.
Polyhedral 3D structure of human plasma very low density lipoproteins by individual particle cryo-electron tomography1.
CGI-58 facilitates the mobilization of cytoplasmic triglyceride for lipoprotein secretion in hepatoma cells.
Inhibition of phosphatidylcholine synthesis via the phosphatidylethanolamine methylation pathway impairs incorporation of bulk lipids into VLDL in cultured rat hepatocytes.
apoA-IV tagged with the ER retention signal KDEL perturbs the intracellular trafficking and secretion of apoB.
Apolipoprotein B is synthesized in selected human non-hepatic cell lines but not processed into mature lipoprotein
The conversion of apoB100 low density lipoprotein/high density lipoprotein particles to apoB100 very low density lipoproteins in response to oleic acid occurs in the endoplasmic reticulum and not in the Golgi in McA RH7777 cells.
Normal activity of microsomal triglyceride transfer protein is required for the oleate-induced secretion of very low density lipoproteins containing apolipoprotein B from McA-RH7777 cells.
Overexpression of human diacylglycerol acyltransferase 1, acyl-coa:cholesterol acyltransferase 1, or acyl-CoA:cholesterol acyltransferase 2 stimulates secretion of apolipoprotein B-containing lipoproteins in McA-RH7777 cells.
Gamma-Tocotrienol Attenuates the Hepatic Inflammation and Fibrosis by Suppressing Endoplasmic Reticulum Stress in Mice
Heterogeneity of N-linked sugar chains of apolipoprotein B-100 in Watanabe heritable hyperlipidemic and fasting rabbits
Regulation of lipoprotein synthesis. Studies on the molecular mechanisms of lipoprotein synthesis and their regulation by estrogen in the cockerel
Insulin inhibits the maturation phase of VLDL assembly via a phosphoinositide 3-kinase-mediated event
Apolipoprotein A-IV expression in mouse liver enhances triglyceride secretion and reduces hepatic lipid content by promoting very low density lipoprotein particle expansion
How does mutant proprotein convertase neural apoptosis-regulated convertase 1 induce autosomal dominant hypercholesterolemia?
Hepatic VLDL secretion: DGAT1 determines particle size but not particle number, which can be supported entirely by DGAT2.
Increased very low density lipoprotein secretion and gonadal fat mass in mice overexpressing liver DGAT1.
Self-association and lipid binding properties of the lipoprotein initiating domain of apolipoprotein B
Microsomal triglyceride transfer protein activity is not required for the initiation of apolipoprotein B-containing lipoprotein assembly in McA-RH7777 cells
Identification of cysteine pairs within the amino-terminal 5% of apolipoprotein B essential for hepatic lipoprotein assembly and secretion.
The activity of microsomal triglyceride transfer protein is essential for accumulation of triglyceride within microsomes in McA-RH7777 cells. A unified model for the assembly of very low density lipoproteins.
The assembly and secretion of apolipoprotein B-48-containing very low density lipoproteins in McA-RH7777 cells
Microsomal triacylglycerol transfer protein is required for lumenal accretion of triacylglycerol not associated with ApoB, as well as for ApoB lipidation.
Complexity in the secretory pathway: the assembly and secretion of apolipoprotein B-containing lipoproteins.
A Drosophila microsomal triglyceride transfer protein homolog promotes the assembly and secretion of human apolipoprotein B. Implications for human and insect transport and metabolism
A point mutation decouples the lipid transfer activities of microsomal triglyceride transfer protein
Influence of 4-aminopyrazolopyrimidine on morphology, synthesis of triglyceride and protein and their secretion in rat hepatocytes
Inhibition of the microsomal triglyceride transfer protein blocks the first step of apolipoprotein B lipoprotein assembly but not the addition of bulk core lipids in the second step.
Inhibition of secretion of truncated apolipoproteins B by monomethylethanolamine is independent of the length of the apolipoprotein.
Folding of the amino-terminal domain of apolipoprotein B initiates microsomal triglyceride transfer protein-dependent lipid transfer to nascent very low density lipoprotein.
ADP-ribosylation factor 1 and its activation of phospholipase D are important for the assembly of very low density lipoproteins
Intracellular assembly of very low density lipoproteins containing apolipoprotein B100 in rat hepatoma McA-RH7777 cells.
Hepatic glucose production is more sensitive to insulin-mediated inhibition than hepatic VLDL-triglyceride production
A phospholipase D-dependent process forms lipid droplets containing caveolin, adipocyte differentiation-related protein, and vimentin in a cell-free system.
Binding of microsomal triglyceride transfer protein to lipids results in increased affinity for apolipoprotein B: evidence for stable microsomal MTP-lipid complexes
Demonstration of a physical interaction between microsomal triglyceride transfer protein and apolipoprotein B during the assembly of ApoB-containing lipoproteins.
Apolipoprotein B100 exit from the endoplasmic reticulum (ER) is COPII-dependent, and its lipidation to very low density lipoprotein occurs post-ER.
Multivesicular bodies isolated from rat hepatocytes. Cytochemical evidence for transformation into secondary lysosomes by fusion with primary lysosomes
Direct evidence for a two-step assembly of ApoB48-containing lipoproteins in the lumen of the smooth endoplasmic reticulum of rabbit enterocytes
Proteomic and lipid characterization of apolipoprotein B-free luminal lipid droplets from mouse liver microsomes: implications for very low density lipoprotein assembly
Nascent lipidated apolipoprotein B is transported to the Golgi as an incompletely folded intermediate as probed by its association with network of endoplasmic reticulum molecular chaperones, GRP94, ERp72, BiP, calreticulin, and cyclophilin B.
VLDL exits from the endoplasmic reticulum in a specialized vesicle, the VLDL transport vesicle, in rat primary hepatocytes
The assembly of very low density lipoproteins in rat hepatoma McA-RH7777 cells is inhibited by phospholipase A2 antagonists
Golgi-associated maturation of very low density lipoproteins involves conformational changes in apolipoprotein B, but is not dependent on apolipoprotein E
ApoE, Lipids & Cholesterol
Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.