PMID: 9530876Apr 8, 1998Paper

Subcellular localization of beta-1,3-glucanase in Puccinia recondita f.sp. tritici-infected wheat leaves

Planta
G Hu, F H Rijkenberg

Abstract

An antiserum raised against the purified 33-kDa beta-1,3-glucanase of wheat (Triticum aestivum L.) was employed to investigate the ultrastructural localization of the enzyme in wheat leaves infected with Puccinia recondita Rob. ex Desm. f.sp. tritici Eriks. and Henn. using a post-embedding immunogold labelling technique. In both compatible and incompatible interactions, beta-1,3-glucanase was detected in the host plasmalemma and in the domain of the host cell wall near the plasmalemma of the mesophyll cells, but higher concentrations of the enzyme were detected in infected resistant wheat leaves than in infected susceptible ones. beta-1,3-Glucanase was also found in the secondary thickening of xylem vessels and in the walls of guard cells, epidermal cells and phloem elements, while no labelling was observed in host organelles, viz. vacuoles, mitochondria, endoplasmic reticulum, Golgi bodies, nuclei and chloroplasts. A low concentration of the enzyme was detected on the intercellular hyphal wall and in the hyphal cytoplasm. In the compatible interaction, beta-1,3-glucanase was demonstrated to accumulate predominantly in the haustorial wall and extrahaustorial matrix. In the incompatible interaction, strong labelling for beta-1,3...Continue Reading

Citations

Aug 9, 2002·Trends in Plant Science·Kurt Mendgen, Matthias Hahn
Dec 13, 2006·Molecular Plant-microbe Interactions : MPMI·Harrold A van den BurgPierre J G M de Wit
Mar 24, 2007·Molecular Plant-microbe Interactions : MPMI·Torben GjettingMichael F Lyngkjaer
Dec 21, 2007·Biological Reviews of the Cambridge Philosophical Society·Dale R WaltersBruce D L Fitt
Feb 25, 2005·Genome Génome / Conseil National De Recherches Canada·F P HanD J Somers
Feb 3, 2007·The Plant Journal : for Cell and Molecular Biology·Amit LevyBernard L Epel

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