Subcellular Localization of Class I Histone Deacetylases in the Developing Xenopus tectum

Frontiers in Cellular Neuroscience
Xia GuoWanhua Shen

Abstract

Histone deacetylases (HDACs) are thought to localize in the nucleus to regulate gene transcription and play pivotal roles in neurogenesis, apoptosis, and plasticity. However, the subcellular distribution of class I HDACs in the developing brain remains unclear. Here, we show that HDAC1 and HDAC2 are located in both the mitochondria and the nucleus in the Xenopus laevis stage 34 tectum and are mainly restricted to the nucleus following further brain development. HDAC3 is widely present in the mitochondria, nucleus, and cytoplasm during early tectal development and is mainly distributed in the nucleus in stage 45 tectum. In contrast, HDAC8 is broadly located in the mitochondria, nucleus, and cytoplasm during tectal development. These data demonstrate that HDAC1, HDAC2, and HDAC3 are transiently localized in the mitochondria and that the subcellular distribution of class I HDACs in the Xenopus tectum is heterogeneous. Furthermore, we observed that spherical mitochondria accumulate in the cytoplasm at earlier stages, whereas elongated mitochondria are evenly distributed in the tectum at later stages. The activity of histone acetylation (H4K12) remains low in mitochondria during tectal development. Pharmacological blockades of HDACs...Continue Reading

References

Jan 1, 1988·Annual Review of Cell Biology·G Attardi, G Schatz
Apr 1, 1972·Developmental Biology·J W Chase, I B Dawid
Jan 1, 1994·Current Topics in Developmental Biology·C B Chien, W A Harris
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·S EmilianiE Verdin
Jan 19, 2000·Nature·B D Strahl, C D Allis
Aug 10, 2000·Trends in Cell Biology·S Desagher, J C Martinou
Oct 21, 2000·Human Reproduction·A H Sathananthan, A O Trounson
Jul 24, 2001·The Journal of Biological Chemistry·W FischleE Verdin
Aug 22, 2001·Molecular Biology of the Cell·E SmirnovaA M van der Bliek
Jan 10, 2002·The Journal of Biological Chemistry·Wen-Ming YangEdward Seto
Nov 14, 2002·The Biochemical Journal·Annemieke J M de RuijterAndré B P van Kuilenburg
Dec 25, 2002·The Journal of Cell Biology·Mariusz KarbowskiRichard J Youle
Feb 19, 2004·The Journal of Biological Chemistry·Lorena GriparicAlexander M van der Bliek
Mar 17, 2004·Molecular Cell·Haim Y CohenDavid A Sinclair
Apr 13, 2004·Journal of Cell Science·David A SmillieJohn Sommerville
Mar 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Chitra SubramanianRoland P S Kwok
May 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Haidong GuBernard Roizman
Nov 16, 2005·Annual Review of Genetics·Koji Okamoto, Janet M Shaw
May 18, 2006·Annual Review of Cell and Developmental Biology·David C Chan
Sep 7, 2006·Nature Reviews. Drug Discovery·Jessica E BoldenRicky W Johnstone
Jan 19, 2007·Nature Clinical Practice. Cardiovascular Medicine·Susan ChungAndre Terzic
Apr 10, 2007·Journal of Molecular Neuroscience : MN·Ron S BroideChristopher J Winrow
Oct 12, 2007·Nature Reviews. Molecular Cell Biology·Scott A Detmer, David C Chan
Oct 26, 2007·Trends in Cell Biology·Kara L CervenyHiromi Sesaki
Feb 23, 2008·Nature Reviews. Molecular Cell Biology·Xiang-Jiao Yang, Edward Seto
Dec 17, 2008·Neuron·Mark P MattsonAiwu Cheng
Apr 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rusty L MontgomeryEric N Olson
Jun 26, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mohd W AkhtarLisa M Monteggia
Oct 8, 2009·Human Molecular Genetics·Hsiuchen Chen, David C Chan
Feb 9, 2010·Nature Chemical Biology·James E BradnerRalph Mazitschek
Mar 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Swati GuptaFarah D Lubin
Apr 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Oliver M DoveyShaun M Cowley
Sep 17, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ludmila A VolobouevaRona G Giffard
Oct 20, 2010·ASN Neuro·Aiwu ChengMark P Mattson
Jul 29, 2011·Journal of Neurophysiology·Wanhua ShenHollis T Cline
Sep 20, 2011·Journal of Cellular Physiology·Jee Suk LeeYoung Hyun Yoo

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
electron microscopy
acetylation
histone acetylation
nuclear translocation

Software Mentioned

Adobe Photoshop
iMaris

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis