PMID: 9556757Aug 1, 1997Paper

Subcellular localization of hepatitis C virus structural proteins in the liver of transgenic mice

Japanese Journal of Medical Science & Biology
K MoriyaK Koike

Abstract

Hepatitis C virus (HCV) core and envelope proteins are suggested to be responsible for the pathogenesis of hepatic and extrahepatic manifestations in chronic hepatitis C. Moreover, the core protein is implicated in the regulation of the transcription of cellular genes including c-myc, RB and p53. Determining the subcellular localization of the core and envelope proteins is therefore necessary to elucidate their behaviors, particularly in vivo ones, regarding the interaction with transcriptional regulatory proteins or gene elements. We defined the subcellular localization of HCV envelope and core proteins which were expressed in substantial levels in the liver of transgenic mice. Subcellular fractionation by ultra-centrifugation revealed that the envelope proteins were present principally in the microsomes of the liver, while a small amount of the protein was detected also in the nuclei. Immunohistochemistry confirmed the localization of envelope proteins in the nuclei. In contrast, the core protein was detected principally in the cytoplasmic fraction, where it was closely associated with lipids. A low level of the core protein was detected also in the nuclei and microsomal fraction. These results suggest possible interaction of...Continue Reading

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Citations

Feb 20, 2009·Journal of Gastroenterology·Kazuhiko Koike
Jun 24, 2009·Journal of Hepatology·Birke BartoschFabien Zoulim
Nov 24, 2011·Virology Journal·Shah JahanSajida Hassan
Sep 20, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Kazuhiko Koike
Apr 11, 2008·Journal of Gastroenterology and Hepatology·Kazuhiko KoikeKyoji Moriya
May 28, 2004·Journal of Virology·Kiyoko OkamotoYoshiharu Matsuura

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