Subcellular localization of human voltage-dependent anion channel isoforms.

The Journal of Biological Chemistry
W H YuM Forte

Abstract

The voltage-dependent anion channel of the outer mitochondrial membrane, VDAC (also known as mitochondrial porin), is a small abundant protein which forms a voltage-gated pore when incorporated into planar lipid bilayers. This protein forms the primary pathway for movement of major metabolites through the outer membrane. Recently, it has been demonstrated that two human VDAC genes, HVDAC1 and HVDAC2, produce three proteins that differ most significantly at their amino termini. These results suggest that the distinct amino termini lead to the targeting of individual VDAC isoforms to different cellular compartments. Consistent with this hypothesis, recent reports suggest that HIV-DAC1 is found in the plasma membrane of mammalian cells. To define the subcellular location of HVDAC isoforms, HVDAC genes were modified so that the encoded proteins contain COOH-terminal epitopes recognized by either of two monoclonal antibodies. Introduction of these epitope tags had no effect on the function of modified VDAC proteins. Epitope-tagged proteins were then individually expressed in COS7 cells or rat astrocytes and the intracellular location of each isoform subsequently identified by subcellular fractionation, light level immunofluorescence...Continue Reading

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M W McEneryS H Snyder
Mar 10, 1990·European Journal of Biochemistry·S PrehnT A Rapoport
Aug 1, 1989·Acta Physiologica Scandinavica·T JalonenP Arhem
Oct 1, 1989·The Journal of Membrane Biology·M Colombini
Mar 1, 1989·The Histochemical Journal·K T Tokuyasu
Feb 29, 1984·Biochimica Et Biophysica Acta·M LindénB D Nelson
Oct 1, 1984·Journal of Ultrastructure Research·G GriffithsJ Dubochet
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·R DermietzelD C Spray
Feb 1, 1995·Journal of Bioenergetics and Biomembranes·S A KonstantinovaD B Zorov
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·M C Sorgato, O Moran

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Citations

Aug 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M Kozak
Jul 2, 2003·FEBS Letters·Robert W GilkersonRoderick A Capaldi
Nov 25, 2000·The International Journal of Biochemistry & Cell Biology·C SchwarzerN Hilschmann
May 16, 2002·The International Journal of Biochemistry & Cell Biology·Christian SchwarzerNorbert Hilschmann
Jun 5, 1998·The International Journal of Biochemistry & Cell Biology·B GuibertD Siemen
Sep 14, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Karima BettayebMarc Flajolet
Dec 12, 2012·BMC Medical Genomics·Jianping LiuZhuchu Chen
Apr 1, 1996·Journal of Bioenergetics and Biomembranes·M HuizingU Wendel
Oct 13, 2004·The Journal of General Physiology·Seiko F OkadaRichard C Boucher
Apr 1, 1996·Journal of Bioenergetics and Biomembranes·W H Yu, M Forte
Feb 27, 2010·FEBS Letters·Vito De PintoAlfons Lawen
Mar 13, 2012·Mitochondrion·Shinya MatsumotoDongchon Kang
Mar 30, 2010·Molecular Aspects of Medicine·Varda Shoshan-BarmatzNir Arbel
Mar 8, 2008·Peptides·Xin-Peng DunZheng-Wang Chen
Feb 10, 2007·The FEBS Journal·Xin-Peng DunTomas Bergman
Oct 12, 2011·Biochimica Et Biophysica Acta·Ravshan Z Sabirov, Petr G Merzlyak
Oct 25, 2000·Mechanisms of Development·K Wertz, B G Herrmann
Jul 15, 2018·The Journal of Biological Chemistry·Ligia Elena González-SerranoMarie Sissler
Sep 13, 2007·Journal of Cellular Physiology·Robert A Maue
Mar 1, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·D P PooleJ B Furness
Mar 29, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·FrancesaA De GiorgiFrançois Ichas
Nov 28, 2000·Plant & Cell Physiology·F J CorpasR N Trelease
Oct 24, 2003·The Journal of Biological Chemistry·Mark A BakerAlfons Lawen
May 18, 1999·The American Journal of Physiology·S Vayro, M Silverman
Jul 18, 2017·Frontiers in Physiology·Amadou K S CamaraWai-Meng Kwok
May 20, 2020·Proceedings of the National Academy of Sciences of the United States of America·Kelvin F ChoAlice Y Ting
Mar 11, 2020·European Biophysics Journal : EBJ·Chetan Malik, Subhendu Ghosh
Feb 9, 2017·The Journal of Cell Biology·Ken-Ichiro HosoiYukio Fujiki

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