Subcellular localization of proteins labeled with GFP in Xanthomonas citri ssp. citri: targeting the division septum

FEMS Microbiology Letters
Paula M M MartinsHenrique Ferreira

Abstract

Xanthomonas citri ssp. citri (Xac) is the causal agent of citrus canker, an economically important disease that affects citrus worldwide. To initiate the characterization of essential biological processes of Xac, we constructed integrative plasmids for the ectopic expression of green fluorescent protein (GFP)-labeled proteins within this bacterium. Here, we show that the disruption of the alpha-amylase gene (amy), the site of plasmid integration into the bacterial chromosome, does not alter its pathogenesis while abolishing completely the ability of Xac to degrade starch. Furthermore, our GFP expression system was used to characterize ORF XAC3408, a hypothetical protein encoded by Xac that shares significant homology to the FtsZ-stabilizing factor ZapA from Bacillus subtilis (ZapA(Bsu)). GFP-XAC3408 expressed in Xac exhibited a septal localization pattern typical of GFP-ZapA(Bsu), which indicates that XAC3408 is the Xac orthologue of the cell division protein ZapA(Bsu). The results demonstrate the potential of GFP labeling for protein functional characterizations in Xac, and, in addition, the Xac mutant strain labeled at the septum constitutes a biological model for the exploration of antibacterial compounds able to inhibit cel...Continue Reading

References

Oct 9, 2002·Genes & Development·Frederico J Gueiros-Filho, Richard Losick
Oct 7, 2003·FEBS Letters·Leonor M P Galvão-BottonAna Paula Valente
Dec 13, 2003·Current Microbiology·Angela Mehta, Yoko B Rosato
Aug 4, 2004·Journal of Molecular Biology·Harry H LowJan Löwe
Dec 22, 2006·Systematic and Applied Microbiology·Norman W SchaadAnne K Vidaver
Sep 20, 2008·Science·David J HaydonLloyd G Czaplewski
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·John N WernerZemer Gitai
Aug 8, 2009·Expert Opinion on Therapeutic Targets·Sonia Kapoor, Dulal Panda

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Citations

Oct 30, 2012·Journal of Bacteriology·I C SilvaH Ferreira
Mar 19, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Giordanni C DantasHenrique Ferreira
Dec 15, 2015·MicrobiologyOpen·Júlia A P SumaresHenrique Ferreira
Dec 18, 2013·MicrobiologyOpen·Amanda P UcciHenrique Ferreira
Mar 28, 2017·Plasmid·Lilian A LacerdaHenrique Ferreira
Mar 12, 2018·Archives of Microbiology·Abigail SaviettoHenrique Ferreira
May 27, 2020·PLoS Pathogens·William CenensChuck S Farah
Jun 26, 2020·Frontiers in Microbiology·Miguel Octavio Pérez NavarroGaldino Andrade
Aug 5, 2017·Frontiers in Microbiology·André S G LorenzoniDirk-Jan Scheffers
Oct 17, 2020·Journal of the Science of Food and Agriculture·Guilherme DilarriHenrique Ferreira
May 20, 2021·Journal of Applied Microbiology·M N CaccalanoH Ferreira
Oct 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luana G MorãoDirk-Jan Scheffers

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