Subcellular localization of the steroid receptor coactivators (SRCs) and MEF2 in muscle and rhabdomyosarcoma cells

Molecular Endocrinology
S L ChenG E Muscat

Abstract

Skeletal muscle differentiation and the activation of muscle-specific gene expression are dependent on the concerted action of the MyoD family and the MADS protein, MEF2, which function in a cooperative manner. The steroid receptor coactivator SRC-2/GRIP-1/TIF-2, is necessary for skeletal muscle differentiation, and functions as a cofactor for the transcription factor, MEF2. SRC-2 belongs to the SRC family of transcriptional coactivators/cofactors that also includes SRC-1 and SRC-3/RAC-3/ACTR/AIB-1. In this study we demonstrate that SRC-2 is essentially localized in the nucleus of proliferating myoblasts; however, weak (but notable) expression is observed in the cytoplasm. Differentiation induces a predominant localization of SRC-2 to the nucleus; furthermore, the nuclear staining is progressively more localized to dot-like structures or nuclear bodies. MEF2 is primarily expressed in the nucleus, although we observed a mosaic or variegated expression pattern in myoblasts; however, in myotubes all nuclei express MEF2. GRIP-1 and MEF2 are coexpressed in the nucleus during skeletal muscle differentiation, consistent with the direct interaction of these proteins. Rhabdomyosarcoma (RMS) cells derived from malignant skeletal muscle t...Continue Reading

Citations

Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hui Joyce LiMichael Mendelsohn
Apr 7, 2005·The Journal of Biological Chemistry·Zhanguo GaoJianping Ye
Feb 11, 2005·Molecular Biology of the Cell·Indumathi Mariappan, Veena K Parnaik
Aug 23, 2002·Molecular and Cellular Biology·Majdi S QutobJoseph Torchia
Dec 18, 2013·The Journal of Clinical Investigation·Lisa E S CroseCorinne M Linardic
Jan 29, 2011·Molecular Endocrinology·Larbi AmazitAnne Guiochon-Mantel
Feb 10, 2009·International Journal of Colorectal Disease·Petros D GrivasHaralabos Kalofonos
Dec 8, 2004·Hormones and Behavior·Erin L O'Bryant, Cynthia L Jordan
Dec 3, 2002·Gene·Olivier Coqueret
Mar 10, 2018·Journal of Applied Genetics·Marcos Vinicius Antunes de LemosFernando Baldi
Aug 28, 2004·Molecular Endocrinology·Julien GrenierCharbel Massaad
Jan 18, 2011·Molecular Endocrinology·S-C Mary WangGeorge E O Muscat
Sep 13, 2003·The Journal of Biological Chemistry·Mario MairaJacques Drouin
Jul 13, 2002·The Journal of Biological Chemistry·Philippe DeleriveChen S Suen
Jun 7, 2003·The Journal of Biological Chemistry·Larbi AmazitAnne Guiochon-Mantel
Feb 3, 2021·The Biochemical Journal·Yung-Jui LinShen-Liang Chen

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