Subcellular localization of VDAC in mitochondria and ER in the cerebellum

Biochimica Et Biophysica Acta
Varda Shoshan-BarmatzNoga Vardi

Abstract

The voltage-dependent anion channel (VDAC) provides passage for adenine nucleotides, Ca2+ and other metabolites into and from mitochondria. Here, the intracellular localization and oligomeric organization of VDAC in brain mitochondria and ER are demonstrated. Immunohistochemical staining of VDAC in rat cerebellum showed high labeling of the Purkinje neurons. Immunogold labeling and EM analysis of the cerebellar molecular layer showed specific VDAC immunostaining of the mitochondrial outer membrane, highly enhanced in contact sites between mitochondria or between mitochondria and associated ER. Purified ER membranes contain VDAC, but not other mitochondrial proteins. Chemical cross-linking of isolated mitochondria, ER or purified VDAC demonstrated the existence of VDAC in oligomeric form. Based on the enrichment of VDAC in the junctional face of closely associated mitochondrial and ER membranes and the existence of VDAC oligomers, we propose an involvement of VDAC in specialized intermembrane communication between mitochondria or between ER and mitochondria, serving to complement the tight structural and functional coupling observed between these organelles.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 1, 1992·Journal of Bioenergetics and Biomembranes·F P Thinnes
Sep 11, 1986·Biochimica Et Biophysica Acta·J KrauseD Brdiczka
Jan 1, 1971·Protoplasma·W W Franke, J Kartenbeck
Dec 7, 1983·Biochimica Et Biophysica Acta·M Lindén, P Gellerfors
Aug 1, 1982·The Anatomical Record·D F MontisanoT W James
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·R DermietzelD C Spray
Feb 1, 1995·Journal of Bioenergetics and Biomembranes·S A KonstantinovaD B Zorov
Jul 17, 1995·Biochimica Et Biophysica Acta·M Zoratti, I Szabò
Jan 1, 1993·The Journal of Physiology·J W BassaniD M Bers
Jan 26, 1996·Biochemical and Biophysical Research Communications·M Lindén, G Karlsson
May 20, 1996·FEBS Letters·V Shoshan-BarmatzL M Heilmeyer
Nov 8, 1996·The Journal of Biological Chemistry·T Rostovtseva, M Colombini
Dec 27, 1996·The Journal of Biological Chemistry·P B Simpson, J T Russell
Apr 1, 1996·Journal of Bioenergetics and Biomembranes·W H Yu, M Forte
Jun 1, 1997·The Journal of Membrane Biology·T Hodge, M Colombini
Jul 25, 1997·The Journal of Biological Chemistry·M J SampsonW J Craigen
Jan 27, 1998·Journal of Structural Biology·G A PerkinsM H Ellisman
Jun 5, 1998·The International Journal of Biochemistry & Cell Biology·B GuibertD Siemen
Jun 30, 1998·Pflügers Archiv : European journal of physiology·S ReymannF P Thinnes
Jul 8, 1998·European Journal of Biochemistry·I ShafirV Shoshan-Barmatz
Nov 24, 1999·The EMBO Journal·G SzalaiG Hajnóczky
May 18, 2000·Biochemical Society Transactions·A P HalestrapA O'Toole
Nov 25, 2000·The International Journal of Biochemistry & Cell Biology·C SchwarzerN Hilschmann
Feb 13, 2001·Trends in Biochemical Sciences·V P Skulachev
Apr 20, 2001·Biochimica Et Biophysica Acta·K D HinschL Konrad
Dec 26, 2001·Biophysical Journal·Tatiana K RostovtsevaMarco Colombini
May 23, 2002·Biochimie·Yoshihide Tsujimoto, Shigeomi Shimizu

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Citations

Jul 9, 2009·Plant Cell Reports·Tomonobu KusanoYoshihiro Takahashi
Dec 19, 2012·Protoplasma·Ashwini GodboleMathew K Mathew
Apr 25, 2007·Apoptosis : an International Journal on Programmed Cell Death·Stefan Grimm, Dieter Brdiczka
Apr 10, 2013·Apoptosis : an International Journal on Programmed Cell Death·Yoshihiro Takahashi, Chika Tateda
Jul 25, 2008·Journal of Bioenergetics and Biomembranes·Varda Shoshan-BarmatzHilal Zaid
Aug 7, 2008·Journal of Bioenergetics and Biomembranes·John G Pastorino, Jan B Hoek
Sep 10, 2013·Nature Communications·Jin-Yi Yang, Wei Yuan Yang
Dec 29, 2009·The Journal of Biological Chemistry·Nir Arbel, Varda Shoshan-Barmatz
Jan 26, 2008·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·C LiY Mi
Oct 13, 2010·Molecular and Cellular Biology·Nurit KeinanVarda Shoshan-Barmatz
Jan 21, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sebastian A BarrientosFelipe A Court
Dec 13, 2012·Biochimica Et Biophysica Acta·Haidar Akl, Geert Bultynck
Mar 30, 2010·Molecular Aspects of Medicine·Varda Shoshan-BarmatzNir Arbel
Apr 25, 2009·The International Journal of Biochemistry & Cell Biology·Carlotta GiorgiPaolo Pinton
Mar 23, 2007·International Journal of Mass Spectrometry·Zhang-Zhi HuCathy Wu
Nov 17, 2007·The European Journal of Neuroscience·Varda Shoshan-BarmatzNoga Vardi
Sep 3, 2011·Immunological Reviews·Michal Caspi Tal, Akiko Iwasaki
Nov 9, 2011·Biochimica Et Biophysica Acta·Kyle S McCommis, Christopher P Baines
Dec 18, 2007·Archives of Biochemistry and Biophysics·Pulak KarSajal Chakraborti
Sep 19, 2006·European Journal of Pharmacology·Senthilkumar DamodaranRichard A Rabin
Jun 25, 2005·Current Opinion in Cell Biology·Tim Levine, Catherine Rabouille
Dec 3, 2014·Biochimica Et Biophysica Acta·Varda Shoshan-BarmatzShambhoo Sharan Tripathi
Apr 2, 2013·Biochimica Et Biophysica Acta·Nurit KeinanVarda Shoshan-Barmatz
Sep 10, 2014·The Cerebellum·Marianna E Jung
Feb 4, 2012·Plant Signaling & Behavior·Chika TatedaYoshihiro Takahashi
Apr 7, 2012·Cell Calcium·Carlotta GiorgiPaolo Pinton
Feb 14, 2012·Biophysical Journal·Viktoria BetaneliPetra Schwille
Apr 28, 2016·Biochimica Et Biophysica Acta·Shamim Naghdi, György Hajnóczky
Jun 13, 2016·European Biophysics Journal : EBJ·Roberta PeruzzoLuigi Leanza
Oct 4, 2016·Frontiers in Synaptic Neuroscience·Seok-Kyu KwonFranck Polleux
Aug 19, 2016·Molecular Biology of the Cell·Edith Honvo-HouétoSandrine Truchet
Mar 13, 2012·International Journal of Molecular Sciences·Benjamin Clémençon
Jun 24, 2017·Cell Death & Disease·Jemma GatliffMichelangelo Campanella
Apr 3, 2014·Physiological Reviews·Ildiko Szabo, Mario Zoratti

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