Subcellular Localization Screening of Colletotrichum higginsianum Effector Candidates Identifies Fungal Proteins Targeted to Plant Peroxisomes, Golgi Bodies, and Microtubules

Frontiers in Plant Science
Guillaume P RobinRichard O'Connell

Abstract

The genome of the hemibiotrophic anthracnose fungus, Colletotrichum higginsianum, encodes a large inventory of putative secreted effector proteins that are sequentially expressed at different stages of plant infection, namely appressorium-mediated penetration, biotrophy and necrotrophy. However, the destinations to which these proteins are addressed inside plant cells are unknown. In the present study, we selected 61 putative effector genes that are highly induced in appressoria and/or biotrophic hyphae. We then used Agrobacterium-mediated transformation to transiently express them as N-terminal fusions with fluorescent proteins in cells of Nicotiana benthamiana for imaging by confocal microscopy. Plant compartments labeled by the fusion proteins in N. benthamiana were validated by co-localization with specific organelle markers, by transient expression of the proteins in the true host plant, Arabidopsis thaliana, and by transmission electron microscopy-immunogold labeling. Among those proteins for which specific subcellular localizations could be verified, nine were imported into plant nuclei, three were imported into the matrix of peroxisomes, three decorated cortical microtubule arrays and one labeled Golgi stacks. Two perox...Continue Reading

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Citations

May 23, 2020·Plants·Peter-Louis Plaumann, Christian Koch
Jan 28, 2019·Applied Microbiology and Biotechnology·Casley Borges de QueirozMarisa Vieira de Queiroz
Jul 26, 2018·International Journal of Molecular Sciences·Yaqin YanLu Zheng
Aug 9, 2020·Molecular Plant Pathology·Claire Kanja, Kim E Hammond-Kosack
Jan 27, 2021·BMC Plant Biology·María E Sánchez-SandovalS M Teresa Hernández-Sotomayor
May 11, 2021·Frontiers in Plant Science·Eva C HenningsenMelania Figueroa
May 17, 2021·Current Opinion in Plant Biology·Melania FigueroaEva C Henningsen
Oct 26, 2020·Current Opinion in Plant Biology·Deepak D Bhandari, Federica Brandizzi
Aug 13, 2021·Journal of Integrative Plant Biology·Muhammad TariqjaveedWenxian Sun

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Datasets Mentioned

BETA
GSE33683

Methods Mentioned

BETA
confocal microscopy
RNA-Seq
ChECs
Destinations
transgenic
GTPases
transmission electron microscopy
nucleotide exchange

Software Mentioned

TopHat2
Genesis
NLStradamus
Fiji
PeroxisomeDB
PTS1Prowler
PredPlantPTS1
NetNES
WoLF PSORT
HTseq

Related Concepts