Subcellular Microanatomy by 3D Deconvolution Brightfield Microscopy: Method and Analysis Using Human Chromatin in the Interphase Nucleus.

Anatomy Research International
Paul Joseph Tadrous

Abstract

Anatomy has advanced using 3-dimensional (3D) studies at macroscopic (e.g., dissection, injection moulding of vessels, radiology) and microscopic (e.g., serial section reconstruction with light and electron microscopy) levels. This paper presents the first results in human cells of a new method of subcellular 3D brightfield microscopy. Unlike traditional 3D deconvolution and confocal techniques, this method is suitable for general application to brightfield microscopy. Unlike brightfield serial sectioning it has subcellular resolution. Results are presented of the 3D structure of chromatin in the interphase nucleus of two human cell types, hepatocyte and plasma cell. I show how the freedom to examine these structures in 3D allows greater morphological discrimination between and within cell types and the 3D structural basis for the classical "clock-face" motif of the plasma cell nucleus is revealed. Potential for further applications discussed.

References

Sep 1, 1972·The British Journal of Surgery·C Oh, A E Kark
Jan 1, 1997·Surgical and Radiologic Anatomy : SRA·P GailloudD A Rüfenacht
Dec 2, 1999·Methods : a Companion to Methods in Enzymology·J G McNallyJ A Conchello
May 5, 2007·Scanning·Flaviana Dornela VerliMaria Antonieta Lopes de Souza
Sep 5, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Jacques RouquetteStanislav Fakan
Jan 26, 2010·Journal of Microscopy·P J Tadrous

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Citations

Jan 16, 2014·Microvascular Research·Sonja SokicEric M Brey

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Methods Mentioned

BETA
dissection
confocal microscopy
light microscopy
fluorescence microscopy
electron microscopy

Software Mentioned

BiaQIm
FATCAM

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