Subcellular phototoxicity of 5-aminolaevulinic acid (ALA)

Lasers in Surgery and Medicine
H LiangM W Berns

Abstract

5-aminolaevulinic acid (ALA) is a new, promising photosensitizer for PDT of cancer. Subcellular toxicity induced by ALA and light exposure in single cells was studied to elucidate the mechanism of cell damage. CPAE, PTK2, and rat neonatal myocardial cells treated with ALA were examined for localization using fluorescence microscopy and for subcellular phototoxicity using 630 nm laser microbeam irradiation of specific subcellular regions. In CPAE and PTK2 cells, a large amount of fluorescence was detected in the peri-nuclear cytoplasm. In rat neonatal myocardial cells, the sensitizer selectively localized in the large mitochondria. In both cell types, there was little phototoxicity when the peripheral cytoplasmic region was exposed, as compared to considerable phototoxicity with exposure of either the perinuclear or nuclear regions. Both the CPAE and PTK2 cells demonstrated that the nucleus followed by the perinuclear cytoplasm are the most sensitive cell areas with no sensitivity in the peripheral cytoplasm.

References

Jul 30, 1992·Journal of Photochemistry and Photobiology. B, Biology·J C Kennedy, R H Pottier
Nov 1, 1991·Photochemistry and Photobiology·D P HeS H Selman
Jun 1, 1989·Journal of Photochemistry and Photobiology. B, Biology·C N Zhou
Nov 1, 1987·Photochemistry and Photobiology·J S NelsonM W Berns
Jan 1, 1993·Science Progress·D Phillips
Dec 1, 1993·Journal of Photochemistry and Photobiology. B, Biology·H SchneckenburgerA Rück
Dec 1, 1993·Photochemistry and Photobiology·T J Dougherty
Apr 1, 1993·Journal of Photochemistry and Photobiology. B, Biology·P Charlesworth, T G Truscott

❮ Previous
Next ❯

Citations

Jul 31, 2010·IEEE Journal of Selected Topics in Quantum Electronics : a Publication of the IEEE Lasers and Electro-optics Society·Brian W PogueKeith D Paulsen
Mar 21, 2001·Biotechnology & Genetic Engineering Reviews·E SobolV Svistushkin
Feb 3, 2004·Lasers in Surgery and Medicine·Tsuimin TsaiChin-Tin Chen
Aug 5, 2004·Photochemistry and Photobiology·Su-Juan Zhang, Zhen-Xi Zhang
Aug 2, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Henriëtte S de BruijnDominic J Robinson
Feb 23, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Alexander S Sobolev
Aug 18, 2000·Immunology and Cell Biology·A A RosenkranzA S Sobolev
Oct 15, 2020·Biochemistry. Biokhimii︠a︡·A A Rosenkranz, T A Slastnikova
Apr 27, 2000·Progress in Biophysics and Molecular Biology·A S SobolevA A Rosenkranz
May 5, 2017·Journal of Photochemistry and Photobiology. B, Biology·Viktorija HercegAndrej Babič
Jul 27, 2001·Journal of Photochemistry and Photobiology. B, Biology·W LiK Hashimoto

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved