PMID: 9182728Jun 1, 1997Paper

Subcellular structure containing mRNA for beta subunit of mitochondrial H+-ATP synthase in rat hepatocytes is translationally active

The Biochemical Journal
J RicartJ M Cuezva

Abstract

We have recently reported that the nuclear-encoded mRNA for the beta subunit of mitochondrial H+-ATP synthase (beta-mRNA) is localized in rounded, electron-dense clusters in the cytoplasm of rat hepatocytes. Clusters of beta-mRNA are often found in close proximity to mitochondria. These findings suggested a role for these structures in controlling the cytoplasmic expression and sorting of the encoded mitochondrial precursor. Here we have addressed the question of whether the structures containing beta-mRNA are translationally active. For this purpose a combination of high-resolution in situ hybridization and immunocytochemical procedures was used. Three different co-localization criteria showed that beta-mRNA-containing structures always revealed positive immunoreactive signals for mitochondrial H+-ATP synthase (F1-ATPase), ribosomal and hsc70 proteins. Furthermore, clusters show evidence in situ of developmental changes in the translational efficiency of the beta-mRNA. These findings suggest that structures containing beta-mRNA are translationally active irrespective of their cytoplasmic location. The immunocytochemical quantification of the cytoplasmic presentation of hsc70 in the hepatocyte reveals that approx. 86% of the pr...Continue Reading

Citations

Jul 8, 2015·RNA Biology·Chen LesnikYoav Arava
Jan 6, 2007·PloS One·Marta Martínez-DiezJosé M Cuezva
Aug 23, 2007·Journal of Bioenergetics and Biomembranes·José M CuezvaAlvaro D Ortega
Jan 8, 2020·Antioxidants & Redox Signaling·Pau B Esparza-Moltó, José M Cuezva
Dec 6, 2011·FEBS Letters·Erez EliyahuYoav Arava
Jul 29, 2010·Journal of Cell Science·Alvaro D OrtegaJosé M Cuezva
Dec 20, 2000·Biochimie·C G VallejoA Rodríguez-Peña
Aug 23, 2002·Biochimica Et Biophysica Acta·Travis Beddoe, Trevor Lithgow

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.