Subcellular topography modulates actin dynamics and signaling in B-cells

Molecular Biology of the Cell
Christina KetchumArpita Upadhyaya

Abstract

B-cell signaling activation is most effectively triggered by the binding of B-cell receptors (BCRs) to membrane-bound antigens. In vivo, B-cells encounter antigen on antigen-presenting cells (APC), which possess complex surfaces with convoluted topographies, a fluid membrane and deformable cell bodies. However, whether and how the physical properties of antigen presentation affect B-cell activation is not well understood. Here we use nanotopographic surfaces that allow systematic variation of geometric parameters to show that surface features on a subcellular scale influence B-cell signaling and actin dynamics. Parallel nanoridges with spacings of 3 microns or greater induce actin intensity oscillations on the ventral cell surface. Nanotopography-induced actin dynamics requires BCR signaling, actin polymerization, and myosin contractility. The topography of the stimulatory surface also modulates the distribution of BCR clusters in activated B-cells. Finally, B-cells stimulated on nanopatterned surfaces exhibit intracellular calcium oscillations with frequencies that depend on topography. Our results point to the importance of physical aspects of ligand presentation, in particular, nanotopography for B-cell activation and antige...Continue Reading

References

Jan 1, 1990·Annual Review of Cell Biology·R W Tsien, R Y Tsien
Nov 30, 1989·Biochemical and Biophysical Research Communications·R L Geahlen, J L McLaughlin
Sep 7, 1995·Annals of the New York Academy of Sciences·A L DeFrancoM R Gold
Feb 4, 1999·Biophysical Journal·P P Mitra, B Pesaran
Oct 19, 2000·Methods in Enzymology·S NarumiyaM Uehata
May 25, 2001·Nature·F D BatistaM S Neuberger
May 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Manabu FujimotoThomas F Tedder
Nov 28, 2002·Molecular Cell·Véronique RolliMichael Reth
Apr 12, 2006·Nature Reviews. Molecular Cell Biology·Viola Vogel, Michael Sheetz
Mar 21, 2007·The Journal of Cell Biology·Pieta K MattilaPekka Lappalainen
Jul 13, 2007·Trends in Immunology·Marc BajénoffRonald N Germain
Jul 27, 2007·Angewandte Chemie·Christopher N LaFrattaRichard A Farrer
Mar 11, 2008·Trends in Cell Biology·Kenneth R Myers, James E Casanova
Nov 26, 2008·Nature·Adam C MartinEric F Wieschaus
Jun 10, 2009·The Journal of Experimental Medicine·Kazuhiro SuzukiJason G Cyster
Oct 31, 2009·Investigative Ophthalmology & Visual Science·Simon A PotChristopher J Murphy
May 4, 2010·Current Opinion in Immunology·Bebhinn Treanor, Facundo D Batista
Jul 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Richard L FeltsSriram Subramaniam
Aug 24, 2010·Immunological Reviews·Facundo D BatistaNaomi E Harwood
Apr 13, 2011·The Journal of Experimental Medicine·Bebhinn TreanorFacundo D Batista
May 31, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chaohong LiuWenxia Song
Mar 6, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chaohong LiuWenxia Song
Mar 6, 2012·Journal of Cell Science·Alexander A SmoligovetsJay T Groves
Jul 7, 2012·PloS One·Simone Köhler, Andreas R Bausch
Jul 31, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Keon Woo KwonJunsang Doh
Sep 22, 2012·Biochemical and Biophysical Research Communications·Chaohong LiuWenxia Song
Jan 22, 2013·Biophysical Journal·Nicole Gorfinkiel
Oct 15, 2013·Immunological Reviews·Wenxia SongArpita Upadhyaya
Nov 14, 2013·PLoS Biology·Chaohong LiuWenxia Song
Nov 26, 2013·Biochimica Et Biophysica Acta·Erik Smedler, Per Uhlén

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Citations

Aug 6, 2019·Advanced Science·Alberto PuliafitoEmiliano Descrovi
Feb 6, 2020·Molecular Biology of the Cell·Rachel M LeeWolfgang Losert
Feb 27, 2021·Acta Biomaterialia·Diana StoychevaEnrico Klotzsch

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Methods Mentioned

BETA
confocal microscopy
Fluorescence

Software Mentioned

MATLAB

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